Gene expression profile of HCT-8 cells following single or co-infections with Cryptosporidium parvum and bovine coronavirus.

Autor: Jiménez-Meléndez A; Department of Paraclinical Sciences (PARAFAG), Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway. Alejandro.jimenez.melendez@nmbu.no., Shakya R; Department of Paraclinical Sciences (PARAFAG), Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Markussen T; Department of Paraclinical Sciences (PARAFAG), Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Robertson LJ; Department of Paraclinical Sciences (PARAFAG), Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Myrmel M; Department of Paraclinical Sciences (PARAFAG), Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Makvandi-Nejad S; Research Group Animal Health, Vaccinology, Norwegian Veterinary Institute, Ås, Norway.; Nykode Therapeutics ASA, Oslo Science Park, Oslo, Norway.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Dec 13; Vol. 13 (1), pp. 22106. Date of Electronic Publication: 2023 Dec 13.
DOI: 10.1038/s41598-023-49488-1
Abstrakt: Among the causative agents of neonatal diarrhoea in calves, two of the most prevalent are bovine coronavirus (BCoV) and the intracellular parasite Cryptosporidium parvum. Although several studies indicate that co-infections are associated with greater symptom severity, the host-pathogen interplay remains unresolved. Here, our main objective was to investigate the modulation of the transcriptome of HCT-8 cells during single and co-infections with BCoV and C. parvum. For this, HCT-8 cells were inoculated with (1) BCoV alone, (2) C. parvum alone, (3) BCoV and C. parvum simultaneously. After 24 and 72 h, cells were harvested and analyzed using high-throughput RNA sequencing. Following differential expression analysis, over 6000 differentially expressed genes (DEGs) were identified in virus-infected and co-exposed cells at 72 hpi, whereas only 52 DEGs were found in C. parvum-infected cells at the same time point. Pathway (KEGG) and gene ontology (GO) analysis showed that DEGs in the virus-infected and co-exposed cells were mostly associated with immune pathways (such as NF-κB, TNF-α or, IL-17), apoptosis and regulation of transcription, with a more limited effect exerted by C. parvum. Although the modulation observed in the co-infection was apparently dominated by the virus, over 800 DEGs were uniquely expressed in co-exposed cells at 72 hpi. Our findings provide insights on possible biomarkers associated with co-infection, which could be further explored using in vivo models.
(© 2023. The Author(s).)
Databáze: MEDLINE
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