Baccharis anomala DC. extract reduces inflammation and attenuates hepatic fibrosis in vivo by decreasing NF-kB and extracellular matrix compounds.

Autor: de Souza Basso B; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Bastos MS; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: matheus.bastos@acad.pucrs.br., Antunes GL; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Matzenbacher LS; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Rodrigues KF; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Garcia MCR; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., de Sousa AC; Department of Biochemistry, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, Rio Grande do Sul, 3, Brazil., Levorse VG; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Luft C; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Tonial GV; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Pavanato GM; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Astarita LV; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., da Silva Melo DA; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., Donadio MVF; Laboratory of Pediatric Physical Activity, Infant Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil., Santarém ER; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil., de Oliveira JR; Laboratory of Cellular Biophysics and Inflammation. School of Health and Life Sciences, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, Rio Grande do Sul, Brazil.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Jan; Vol. 237, pp. 107560. Date of Electronic Publication: 2023 Dec 11.
DOI: 10.1016/j.toxicon.2023.107560
Abstrakt: Baccharis anomala DC. (BA) is a plant species found in the tropical regions of South America and is widely used for its hepatoprotective effects, as well as for the treatment of gastrointestinal diseases. Studies have recently reported its antioxidant and anti-inflammatory potential. BA extract can reverse the activated phenotype of hepatic stellate cells (HSC), which plays a central role in extracellular matrix (ECM) deposition in the development of liver fibrosis. Thus, this study aimed to evaluate the effects of the treatment with BA extract on liver fibrosis in a CCl 4 -induced liver fibrosis model in BALB/c mice. Methanolic extract was obtained from BA leaves, a gas chromatography/mass spectrometry (GC/MS) to detect the compounds present was performed, and then administered by intraperitoneal injection in Balb/C mice at a concentration of 50 and 100 mg/kg together with the administration of CCl 4 for inducing liver fibrosis. After 10 weeks, blood analysis, histopathology, oxidative stress, as well as protein and gene expression in the hepatic tissue were performed. Treatment with BA extract was able to reduce profibrotic markers by reducing the expression of α-SMA and Col-1 proteins, as well as reducing the formation of free radicals and lipid peroxidation. (BA extract showed anti-inflammatory effects in the liver by suppressing NF-kB activation and reducing gene expression of signaling targets (IL-6 and iNOS). The data obtained showed that BA extract has antifibrotic and anti-inflammatory effects.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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