Discerning biomimetic nanozyme electrodes based on g-C 3 N 4 nanosheets and molecularly imprinted polythiophene nanofibers for detecting creatinine in microliter droplets of human saliva.

Autor: Saeed M; Sensors and Diagnostics Lab, School of Chemistry, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan., Saddique Z; Sensors and Diagnostics Lab, School of Chemistry, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan., Mujahid A; Sensors and Diagnostics Lab, School of Chemistry, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan., Afzal A; Sensors and Diagnostics Lab, School of Chemistry, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan. Electronic address: adeel.chem@pu.edu.pk.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Mar 01; Vol. 247, pp. 115899. Date of Electronic Publication: 2023 Dec 06.
DOI: 10.1016/j.bios.2023.115899
Abstrakt: The growing risk of death associated with kidney dysfunction underlines the requirement for a cost-effective and precise point-of-care (POC) diagnostic tool to identify chronic kidney disease (CKD) at an early stage. This work reports the development of a non-invasive POC diagnostic based on cost-efficient, disposable electrodes and in situ-designed biomimetic nanozymes. The nanozymes are composed of graphitic carbon nitride nanosheets (gCN) and creatinine-imprinted polythiophene nanofibers (miPTh). Microscopic analyses reveal porous nanofibrous surface morphology of biomimetic miPTh/gCN nanozymes. Bulk imprinting and the inclusion of conductive gCN nanosheets drastically reduced the charge transfer resistance and improved the electron exchange kinetics at the nanozyme-electrolyte interface. The electrochemical oxidation of creatinine is studied via cyclic voltammetry (CV), and differential pulse voltammetry (DPV), which exhibit excellent creatinine recognition ability of biomimetic miPTh/gCN nanozyme sensors compared to pristine polymeric or non-imprinted nanozymes. The sensor reveals linear response toward 200-1000 nmol L -1 creatinine, high sensitivity (4.27 μA cm -2 nmol -1 L), sub-nanomolar detection limit (340 pmol L -1 ), and excellent selectivity over common salivary analytes. To corroborate its real-world utility, the miPTh/gCN nanozyme sensor shows an impressive 94.8% recovery of spiked creatinine concentrations in microliter droplets of human saliva samples. This disposable sensor reveals great potential in the realm of reliable and efficient non-invasive POC diagnostics for healthcare delivery.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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