DNA Vaccine Encoding the Artificial T-Cell Polyepitope Immunogen of Tick-Borne Encephalitis Virus.

Autor: Kisakov DN; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. def_2003@mail.ru., Antonets DV; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Shaburova EV; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Kisakova LA; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Tigeeva EV; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Yakovlev VA; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Starostina EV; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Borgoyakova MB; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Protopopova EV; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Svyatchenko VA; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Loktev VB; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Rudometov AP; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Ilyichev AA; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Nepomnyashchikh TS; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Karpenko LI; State Research Center of Virology and Biotechnology 'VECTOR', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Nov; Vol. 176 (1), pp. 72-76. Date of Electronic Publication: 2023 Dec 13.
DOI: 10.1007/s10517-023-05970-4
Abstrakt: A promising approach to the development of new means for preventing infection caused by tick-borne encephalitis virus can be DNA vaccines encoding polyepitope T-cell immunogens. A DNA vaccine pVAX-AG4-ub encoding an artificial polyepitope immunogen that includes cytotoxic and T-helper epitopes from the NS1, NS3, NS5, and E proteins of the tick-borne encephalitis virus has been obtained. The developed construct ensured the synthesis of the corresponding mRNAs in transfected eukaryotic cells. Immunization of mice with pVAX-AG4-ub induced the formation of a virus-specific T-cell response providing 50% protection from lethal infection with the virus.
(© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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