Photobiomodulation effects of blue light on osteogenesis are induced by reactive oxygen species.

Autor: Albaqami M; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, 150 W. University Blvd, Melbourne, FL, 32901, USA., Aguida B; UMR8256, CNRS, IBPS, Sorbonne, Université, Paris, France., Pourmostafa A; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, 150 W. University Blvd, Melbourne, FL, 32901, USA., Ahmad M; UMR8256, CNRS, IBPS, Sorbonne, Université, Paris, France., Kishore V; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, 150 W. University Blvd, Melbourne, FL, 32901, USA. vkishore@fit.edu.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2023 Dec 13; Vol. 39 (1), pp. 5. Date of Electronic Publication: 2023 Dec 13.
DOI: 10.1007/s10103-023-03951-7
Abstrakt: Blue light-mediated photobiomodulation (PBM) is a promising approach to promote osteogenesis. However, the underlying mechanisms of PBM in osteogenesis are poorly understood. In this study, a human osteosarcoma cell line (i.e., Saos-2 cells) was subjected to intermittent blue light exposure (2500 µM/m 2 /s, 70 mW/cm 2 , 4.2 J/cm 2 , once every 48 h) and the effects on Saos-2 cell viability, metabolic activity, differentiation, and mineralization were investigated. In addition, this study addressed a possible role of blue light induced cellular oxidative stress as a mechanism for enhanced osteoblast differentiation and mineralization. Results showed that Saos-2 cell viability and metabolic activity were maintained upon blue light exposure compared to unilluminated controls, indicating no negative effects. To the contrary, blue light exposure significantly increased (p < 0.05) alkaline phosphatase activity and Saos-2 cell mediated mineralization. High-performance liquid chromatography (HPLC) assay was used for measurement of reactive oxygen species (ROS) activity and showed a significant increase (p < 0.05) in superoxide (O 2•- ) and hydrogen peroxide (H 2 O 2 ) formed after blue light exposure. Together, these results suggest that the beneficial effects of blue light-mediated PBM on osteogenesis may be induced by controlled release of ROS.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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