| Autor: |
Sukhija N; ICAR-National Dairy Research Institute, Karnal, India., Malik AA; TERI School of Advanced Studies, Delhi, India.; The Energy and Resources Institute, North Eastern Regional Centre, Guwahati, India., Devadasan JM; ICAR-National Dairy Research Institute, Karnal, India., Dash A; ICAR-National Dairy Research Institute, Karnal, India., Bidyalaxmi K; ICAR-National Dairy Research Institute, Karnal, India.; ICAR-National Bureau of Animal Genetic Resources, Karnal, India., Ravi Kumar D; ICAR-National Dairy Research Institute, Karnal, India., Kousalaya Devi M; ICAR-National Dairy Research Institute, Karnal, India., Choudhary A; ICAR-National Dairy Research Institute, Karnal, India., Kanaka KK; ICAR-National Dairy Research Institute, Karnal, India.; ICAR- Indian Institute of Agricultural Biotechnology, Ranchi, India., Sharma R; ICAR-National Bureau of Animal Genetic Resources, Karnal, India., Tripathi SB; TERI School of Advanced Studies, Delhi, India., Niranjan SK; ICAR-National Bureau of Animal Genetic Resources, Karnal, India., Sivalingam J; ICAR-National Bureau of Animal Genetic Resources, Karnal, India., Verma A; ICAR-National Dairy Research Institute, Karnal, India. |
| Abstrakt: |
The peculiarity of Indian cattle lies in milk quality, resistance to diseases and stressors as well as adaptability. The investigation addressed selection signatures in Gir and Tharparkar cattle, belonging to arid ecotypes of India. Double digest restriction-site associated DNA sequencing (ddRAD-seq) yielded nearly 26 million high-quality reads from unrelated seven Gir and seven Tharparkar cows. In all, 19,127 high-quality SNPs were processed for selection signature analysis. An approach involving within-population composite likelihood ratio (CLR) statistics and between-population F ST statistics was used to capture selection signatures within and between the breeds, respectively. A total of 191 selection signatures were addressed using CLR and F ST approaches. Selection signatures overlapping 86 and 73 genes were detected as Gir- and Tharparkar-specific, respectively. Notably, genes related to production (CACNA1D, GHRHR), reproduction (ESR1, RBMS3), immunity (NOSTRIN, IL12B) and adaptation (ADAM22, ASL) were annotated to selection signatures. Gene pathway analysis revealed genes in insulin/IGF pathway for milk production, gonadotropin releasing hormone pathway for reproduction, Wnt signalling pathway and chemokine and cytokine signalling pathway for adaptation. This is the first study where selection signatures are identified using ddRAD-seq in indicine cattle breeds. The study shall help in conservation and leveraging genetic improvements in Gir and Tharparkar cattle. |
