A new wild emmer wheat panel allows to map new loci associated with resistance to stem rust at seedling stage.

Autor: Mastrangelo AM; Research Centre for Cereal and Industrial Crops, Council for Agricultural Research and Economics (CREA), Foggia, Italy., Roncallo P; Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS), Departamento de Agronomía, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca, Argentina., Matny O; Department of Plant Pathology, University of Minnesota, Minneapolis, Minnesota, USA., Čegan R; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic.; Department of Plant Developmental Genetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic., Steffenson B; Department of Plant Pathology, University of Minnesota, Minneapolis, Minnesota, USA., Echenique V; Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS), Departamento de Agronomía, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca, Argentina., Šafář J; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic., Battaglia R; Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics (CREA), Fiorenzuola d'Arda, Italy., Barabaschi D; Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics (CREA), Fiorenzuola d'Arda, Italy., Cattivelli L; Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics (CREA), Fiorenzuola d'Arda, Italy., Özkan H; Faculty of Agriculture, Department of Field Crops, University of Çukurova, Adana, Turkey., Mazzucotelli E; Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics (CREA), Fiorenzuola d'Arda, Italy.
Jazyk: angličtina
Zdroj: The plant genome [Plant Genome] 2023 Dec 12, pp. e20413. Date of Electronic Publication: 2023 Dec 12.
DOI: 10.1002/tpg2.20413
Abstrakt: Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a major wheat disease worldwide. A collection of 283 wild emmer wheat [Triticum turgidum L. subsp. dicoccoides (Körn. ex Asch. & Graebn.) Thell] accessions, representative of the entire Fertile Crescent region where wild emmer naturally occurs, was assembled, genotyped, and characterized for population structure, genetic diversity, and rate of linkage disequilibrium (LD) decay. Then, the collection was employed for mapping Pgt resistance genes, as a proof of concept of the effectiveness of genome-wide association studies in wild emmer. The collection was evaluated in controlled conditions for reaction to six common Pgt pathotypes (TPMKC, TTTTF, JRCQC, TRTTF, TTKSK/Ug99, and TKTTF). Most resistant accessions originated from the Southern Levant wild emmer lineage, with some showing a resistance reaction toward three to six tested races. Association analysis was conducted considering a 12K polymorphic single-nucleotide polymorphisms dataset, kinship relatedness between accessions, and population structure. Eleven significant marker-trait associations (MTA) were identified across the genome, which explained from 17% to up to 49% of phenotypic variance with an average 1.5 additive effect (based on the 1-9 scoring scale). The identified loci were either effective against single or multiple races. Some MTAs colocalized with known Pgt resistance genes, while others represent novel resistance loci useful for durum and bread wheat prebreeding. Candidate genes with an annotated function related to plant response to pathogens were identified at the regions linked to the resistance and defined according to the estimated small LD (about 126 kb), as typical of wild species.
(© 2023 The Authors. The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.)
Databáze: MEDLINE
Externí odkaz: