Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers.

Autor: Ayala R; Molecular Cryo-Electron Microscopy Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, 904-0495, Onna-son, Okinawa, Japan., Moiseenko AV; Faculty of Biology, Lomonosov Moscow State University, 1 Leninskie Gory, Bld. 12, 119234, Moscow, Russia., Chen TH; Molecular Cryo-Electron Microscopy Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, 904-0495, Onna-son, Okinawa, Japan., Kulikov EE; Faculty of Biology, Lomonosov Moscow State University, 1 Leninskie Gory, Bld. 12, 119234, Moscow, Russia.; Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 7/2, 60-Letiya Oktyabrya Ave, 117312, Moscow, Russia., Golomidova AK; Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 7/2, 60-Letiya Oktyabrya Ave, 117312, Moscow, Russia., Orekhov PS; Faculty of Biology, Shenzhen MSU-BIT University, 1 International University Park Dr, Dayun New Town, Longgang District, Shenzhen, 518172, China., Street MA; Molecular Cryo-Electron Microscopy Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, 904-0495, Onna-son, Okinawa, Japan., Sokolova OS; Faculty of Biology, Lomonosov Moscow State University, 1 Leninskie Gory, Bld. 12, 119234, Moscow, Russia. sokolova@mail.bio.msu.ru.; Faculty of Biology, Shenzhen MSU-BIT University, 1 International University Park Dr, Dayun New Town, Longgang District, Shenzhen, 518172, China. sokolova@mail.bio.msu.ru., Letarov AV; Faculty of Biology, Lomonosov Moscow State University, 1 Leninskie Gory, Bld. 12, 119234, Moscow, Russia. letarov@gmail.com.; Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 7/2, 60-Letiya Oktyabrya Ave, 117312, Moscow, Russia. letarov@gmail.com., Wolf M; Molecular Cryo-Electron Microscopy Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, 904-0495, Onna-son, Okinawa, Japan. matthias.wolf@oist.jp.; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, 115, Taipei, 15, Taiwan. matthias.wolf@oist.jp.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Dec 11; Vol. 14 (1), pp. 8205. Date of Electronic Publication: 2023 Dec 11.
DOI: 10.1038/s41467-023-43824-9
Abstrakt: The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral tail fibers (LTF). Considerable portions of T5-like phages remain structurally uncharacterized. Here, we present the structure of DT57C determined by cryo-EM, and an atomic model of the virus, which was further explored using all-atom molecular dynamics simulations. The structure revealed a unique way of LTF attachment assisted by a dodecameric collar protein LtfC, and an unusual composition of the phage neck constructed of three protein rings. The tape measure protein (TMP) is organized within the tail tube in a three-stranded parallel α-helical coiled coil which makes direct contact with the genomic DNA. The presence of the C-terminal fragment of the TMP that remains within the tail tip suggests that the tail tip complex returns to its original state after DNA ejection. Our results provide a complete atomic structure of a T5-like phage, provide insights into the process of DNA ejection as well as a structural basis for the design of engineered phages and future mechanistic studies.
(© 2023. The Author(s).)
Databáze: MEDLINE
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