Carbon dioxide as key player in chain elongation and growth of Clostridium kluyveri: Insights from batch and bioreactor studies.

Autor: Fernández-Blanco C; Chemical Engineering Laboratory, Faculty of Sciences and Interdisciplinary Centre of Chemistry and Biology, Centro Interdisciplinar de Química e Bioloxía (CICA), BIOENGIN Group, University of A Coruña, E-15008-A Coruña, Spain., Veiga MC; Chemical Engineering Laboratory, Faculty of Sciences and Interdisciplinary Centre of Chemistry and Biology, Centro Interdisciplinar de Química e Bioloxía (CICA), BIOENGIN Group, University of A Coruña, E-15008-A Coruña, Spain., Kennes C; Chemical Engineering Laboratory, Faculty of Sciences and Interdisciplinary Centre of Chemistry and Biology, Centro Interdisciplinar de Química e Bioloxía (CICA), BIOENGIN Group, University of A Coruña, E-15008-A Coruña, Spain. Electronic address: Kennes@udc.es.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Feb; Vol. 394, pp. 130192. Date of Electronic Publication: 2023 Dec 09.
DOI: 10.1016/j.biortech.2023.130192
Abstrakt: Chain elongation technology allows medium-chain fatty acids (MCFAs) production as an alternative to fossil resources. Clostridium kluyveri generates n-caproate primarily from ethanol and acetate, presumably requiring CO 2 for growth. Here, the impact of CO 2 on C. kluyveri was explored. Bottle studies revealed the bacterium's adaptability to low CO 2 levels, even in conditions with minimal dissolved NaHCO 3 (0.0003 M) and unfavorable pH (below 6) under 1 bar CO 2 . Bioreactor investigations demonstrated a direct correlation between CO 2 availability and bacterial growth. The highest n-caproate production (11.0 g/L) with 90.1 % selectivity was achieved in a bioreactor with continuous CO 2 supply at 3 mL/min. Additional bottle experiments pressurized with 1 bar CO 2 and varying ethanol:acetate ratios (1:1, 2:1, 4:1) also confirmed CO 2 consumption by C. kluyveri. However, increasing the ethanol:acetate ratio did not enhance n-caproate selectivity, likely due to overly acidic pH conditions. These findings provide insights into chain-elongators responses under diverse conditions.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Christian Kennes reports financial support was provided by Spain Ministry of Science and Innovation. Maria C. Veiga reports financial support was provided by Government of Galicia.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: