Recent advances in expression and purification strategies for plant made vaccines.

Autor: Venkataraman S; Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada., Khan I; Forte Protein, Center for Life Science Ventures, Cornell University, Ithaca, NY, United States., Habibi P; Forte Protein, Center for Life Science Ventures, Cornell University, Ithaca, NY, United States., Le M; Forte Protein, Center for Life Science Ventures, Cornell University, Ithaca, NY, United States.; Department of Food Science, Cornell University, Ithaca, NY, United States., Lippert R; Forte Protein, Center for Life Science Ventures, Cornell University, Ithaca, NY, United States., Hefferon K; Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.; Forte Protein, Center for Life Science Ventures, Cornell University, Ithaca, NY, United States.; Department of Microbiology, Cornell University, Ithaca, NY, United States.
Jazyk: angličtina
Zdroj: Frontiers in plant science [Front Plant Sci] 2023 Nov 23; Vol. 14, pp. 1273958. Date of Electronic Publication: 2023 Nov 23 (Print Publication: 2023).
DOI: 10.3389/fpls.2023.1273958
Abstrakt: Plants have been explored as a platform to produce pharmaceutical proteins for over 20 years. Important features such as the cost-effectiveness of production, the ease of scaling up to manufacturing capacity, the lack of cold chain requirements and the ability to produce complex therapeutic proteins which are biologically and functionally identical to their mammalian counterparts, make plants a strong alternative for vaccine production. This review article focuses on both the expression as well as the downstream purification processes for plant made vaccines. Expression strategies including transgenic, transient and cell suspension cultures are outlined, and various plant tissues targeted such as leaves and seeds are described. The principal components used for downstream processing of plant made vaccines are examined. The review concludes with a reflection of the future benefits of plant production platforms for vaccine production.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
(Copyright © 2023 Venkataraman, Khan, Habibi, Le, Lippert and Hefferon.)
Databáze: MEDLINE