The enzymatic synthesis of lactose caprate using Candida rugosa lipase immobilized into ZIF-8 and investigation of its anticancer applications against K562 leukemia and HeLa cancer cells.

Autor: Karimian E; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Marandi A; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Kardanpour R; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Rafiei S; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Amirghofran Z; Immunology Department, Autoimmune Diseases Research Centre, Shiraz University of Medical Sciences Shiraz Iran., Tangestaninejad S; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Moghadam M; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Mirkhani V; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir., Mohammadpoor-Baltork I; Department of Chemistry, Catalysis Division, University of Isfahan Isfahan 81746-73441 Iran stanges@sci.ui.ac.ir moghadamm@sci.ui.ac.ir.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2023 Dec 06; Vol. 13 (50), pp. 35639-35647. Date of Electronic Publication: 2023 Dec 06 (Print Publication: 2023).
DOI: 10.1039/d3ra04632j
Abstrakt: In this study, a lactose fatty acid ester was enzymatically synthesised using immobilized Candida rugosa lipase (CRL). Its anticancer property against K562 leukemia and HeLa cancer cells was carefully investigated. In the first step, a de novo strategy was applied to encapsulate CRL into a microporous zeolite imidazolate framework called ZIF-8. Various characterization techniques including powder X-ray diffraction, Fourier transform infrared spectroscopy, N 2 adsorption-desorption, field-emission scanning electron microscopy and thermogravimetric analysis were used to prove the successful encapsulation of CRL molecules during the formation of ZIF-8 crystals with an enzyme loading of 98% of initial CRL. The effect of various factors such as pH and temperature, affecting the enzymatic activity and reusability of the CRL@ZIF-8 composite were assessed against the free enzyme. Additionally, enzyme catalysis parameters, such as K m and V max , were also assessed. The obtained biocatalyst showed excellent activity in a wide pH range of 2-9 and a temperature range of 30-60 °C. According to the experimental results, the CRL@ZIF-8 composite maintained about 63% of its initial activity after 6 cycles of use. In the next step, the synthesized catalyst was applied for the synthesis of lactose caprate via enzymatic esterification of lactose with capric acid. Further experiments were performed to obtain the cytotoxicity profile of the new derivative. The growth inhibitory effect of the produced lactose caprate on K562 leukemia and HeLa cancer cells determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed its potential anticancer effects against both cell lines (IC 50 , 49.6 and 57.2 μg mL -1 ). Our results indicate that lactose caprate might be a promising candidate for further studies on K562 leukemia and HeLa cancer cells owing to its possible therapeutic usefulness.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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