In situ lipidomics of Staphylococcus aureus osteomyelitis using imaging mass spectrometry.

Autor:	Good CJ; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37235, USA.; Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA., Butrico CE; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Colley ME; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37235, USA.; Department of Biochemistry, Vanderbilt University, Nashville, TN 37235, USA., Gibson-Corley KN; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Cassat JE; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA.; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Spraggins JM; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37235, USA.; Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.; Department of Biochemistry, Vanderbilt University, Nashville, TN 37235, USA.; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37235, USA., Caprioli RM; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37235, USA.; Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.; Department of Biochemistry, Vanderbilt University, Nashville, TN 37235, USA.; Department of Medicine, Vanderbilt University, Nashville, TN 37235, USA.; Department of Pharmacology, Vanderbilt University, Nashville, TN 37235, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Dec 02. Date of Electronic Publication: 2023 Dec 02.
DOI:	10.1101/2023.12.01.569690
Abstrakt:	Osteomyelitis occurs when Staphylococcus aureus invades the bone microenvironment, resulting in a bone marrow abscess with a spatially defined architecture of cells and biomolecules. Imaging mass spectrometry and microscopy are invaluable tools that can be employed to interrogate the lipidome of S. aureus -infected murine femurs to reveal metabolic and signaling consequences of infection. Here, nearly 250 lipids were spatially mapped to healthy and infection-associated morphological features throughout the femur, establishing composition profiles for tissue types. Ether lipids and arachidonoyl lipids were significantly altered between cells and tissue structures in abscesses, suggesting their roles in abscess formation and inflammatory signaling. Sterols, triglycerides, bis(monoacylglycero)phosphates, and gangliosides possessed ring-like distributions throughout the abscess, indicating dysregulated lipid metabolism in a subpopulation of leukocytes that cannot be discerned with traditional microscopy. These data provide chemical insight into the signaling function and metabolism of cells in the fibrotic border of abscesses, likely characteristic of lipid-laden macrophages. Competing Interests: The authors declare no competing financial interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu