Manganese(II) ions suppress the transcription of the citrate exporter encoding gene cexA in Aspergillus niger .

Autor:	Reinfurt A; Research Group Biochemistry, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Vienna, Austria., Fritsche S; Research Group Biochemistry, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Vienna, Austria., Bíró V; Department of Biochemical Engineering, Faculty of Science & Technology, University of Debrecen, Debrecen, Hungary.; Juhász-Nagy Pál Doctoral School of Biology and Environmental Sciences, University of Debrecen, Debrecen, Hungary., Márton A; Department of Biochemical Engineering, Faculty of Science & Technology, University of Debrecen, Debrecen, Hungary.; Juhász-Nagy Pál Doctoral School of Biology and Environmental Sciences, University of Debrecen, Debrecen, Hungary., Ellena V; Research Group Biochemistry, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Vienna, Austria., Fekete E; Department of Biochemical Engineering, Faculty of Science & Technology, University of Debrecen, Debrecen, Hungary., Sándor E; Institute of Food Science, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Debrecen, Hungary., Karaffa L; Department of Biochemical Engineering, Faculty of Science & Technology, University of Debrecen, Debrecen, Hungary., Steiger MG; Research Group Biochemistry, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Vienna, Austria.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2023 Nov 22; Vol. 11, pp. 1292337. Date of Electronic Publication: 2023 Nov 22 (Print Publication: 2023).
DOI:	10.3389/fbioe.2023.1292337
Abstrakt:	Aspergillus niger is an important filamentous fungus used for the industrial production of citric acid. One of the most important factors that affect citric acid production is the concentration of manganese(II) ions present in the culture broth. Under manganese(II)-limiting conditions, the fungus develops a pellet-like morphology that is crucial for high citric acid accumulation. The impact of manganese(II) ions on the transcription of the major citrate exporter encoding gene cexA was studied under manganese(II)-deficient and -sufficient conditions. Furthermore, citric acid production was analyzed in overexpression mutant strains of cexA in the presence and absence of manganese(II) ions, and the influence of CexA on fungal morphology was investigated by microscopy. Transcriptional upregulation of cexA in the absence of manganese(II) ions was observed and, by decoupling cexA expression from the native promoter system, it was possible to secrete more citric acid even in the presence of manganese. This effect was shown for both an inducible and a constitutive overexpression of cexA . Furthermore, it was found that the presence of CexA influences fungal morphology and promotes a more branched phenotype. According to this study, manganese(II) ions suppress transcription of the citrate exporter cexA in Aspergillus niger , causing citric acid secretion to decrease. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Reinfurt, Fritsche, Bíró, Márton, Ellena, Fekete, Sándor, Karaffa and Steiger.)
Databáze:	MEDLINE
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