Wastewater analysis of Mpox virus in a city with low prevalence of Mpox disease: an environmental surveillance study.

Autor:	Oghuan J; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Chavarria C; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Vanderwal SR; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Gitter A; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA.; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA., Ojaruega AA; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Monserrat C; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Bauer CX; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA.; Department of Biostatistics and Data Science, School of Public Health, University of Texas Health Science Center at Houston, TX, USA.; Center of Spatial-temporal Modeling of Applications in Population Sciences, Houston, TX, USA., Brown EL; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Cregeen SJ; The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA., Deegan J; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA.; School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Hanson BM; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA.; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA., Tisza M; The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA., Ocaranza HI; City of El Paso Department of Public Health, El Paso, TX, USA., Balliew J; El Paso Water, El Paso, TX, United States., Maresso AW; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA.; TAILOR Labs, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, 77030, USA., Rios J; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA.; School of Public Health, University of Texas Health Science Center at Houston, TX, USA., Boerwinkle E; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA.; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA., Mena KD; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA.; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA., Wu F; Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health, University of Texas Health Science Center at Houston, TX, USA.; Texas Epidemic Public Health Institute (TEPHI), UTHealth Houston, Houston, TX, USA.
Jazyk:	angličtina
Zdroj:	Lancet regional health. Americas [Lancet Reg Health Am] 2023 Nov 24; Vol. 28, pp. 100639. Date of Electronic Publication: 2023 Nov 24 (Print Publication: 2023).
DOI:	10.1016/j.lana.2023.100639
Abstrakt:	Background: Tracking infectious diseases at the community level is challenging due to asymptomatic infections and the logistical complexities of mass surveillance. Wastewater surveillance has emerged as a valuable tool for monitoring infectious disease agents including SARS-CoV-2 and Mpox virus. However, detecting the Mpox virus in wastewater is particularly challenging due to its relatively low prevalence in the community. In this study, we aim to characterize three molecular assays for detecting and tracking the Mpox virus in wastewater from El Paso, Texas, during February and March 2023. Methods: In this study, a combined approach utilizing three real-time PCR assays targeting the C22L, F3L, and F8L genes and sequencing was employed to detect and track the Mpox virus in wastewater samples. The samples were collected from four sewersheds in the City of El Paso, Texas, during February and March 2023. Wastewater data was compared with reported clinical case data in the city. Findings: Mpox virus DNA was detected in wastewater from all the four sewersheds, whereas only one Mpox case was reported during the sampling period. Positive signals were still observed in multiple sewersheds after the Mpox case was identified. Higher viral concentrations were found in the pellet than in the supernatant of wastewater. Notably, an increasing trend in viral concentration was observed approximately 1-2 weeks before the reporting of the Mpox case. Further sequencing and epidemiological analysis provided supporting evidence for unreported Mpox infections in the city. Interpretation: Our analysis suggests that the Mpox cases in the community is underestimated. The findings emphasize the value of wastewater surveillance as a public health tool for monitoring infectious diseases even in low-prevalence areas, and the need for heightened vigilance to mitigate the spread of Mpox disease for safeguarding global health. Funding: Center of Infectious Diseases at UTHealth, the University of Texas System, and the Texas Epidemic Public Health Institute. The content of this paper is solely the responsibility of the authors and does not necessarily represent the official views of these funding organizations. Competing Interests: The authors declare no competing interest. (© 2023 The Author(s).)
Databáze:	MEDLINE
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