Development of a new broad-spectrum microencapsulation-based spray drying formulation of Bacillus thuringiensis subsp. kurstaki IMBL-B9 for the control of moths.

Autor:	Duraisamy K; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea., Yu NH; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea., Kim SH; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea., Baek JH; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea., Son JY; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea., Choi E; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea., Park MG; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea., Kim J; Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of Korea., Choi JY; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea., Sang MK; Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of Korea., Je YH; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.; Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea., Kim JC; Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Gwangju, Republic of Korea.; Department of Agricultural Chemistry and Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Nov 22; Vol. 14, pp. 1273725. Date of Electronic Publication: 2023 Nov 22 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1273725
Abstrakt:	Certain Bacillus thuringiensis (Bt) strains such as Bt subsp. kurstaki and Bt subsp. aizawai have been widely used for pest management in agricultural practices. However, each strain only shows high specificity for pest control against a narrow range of lepidopteran species, and numerous lepidopteran pests have developed resistance to commercialized Bt strains. Therefore, there is a need for the development of novel Bt bioinsecticides which allow for potent and broad-spectrum insecticidal activity against lepidopteran species, including Spodoptera spp. (Noctuidae) and Plutella xylostealla (Plutellidae). In order to develop a novel bioinsecticide using Bt subsp. kurstaki IMBL-B9 (Btk IMBL-B9) that exhibits excellent insecticidal activity against three different lepidopteran species, we have developed a viable microencapsulation-based spray drying Btk IMBL-B9 formulation. The spore-crystal complex of Btk IMBL-B9 was microencapsulated using coating materials such as gum arabic, maltodextrin, and corn starch via spray drying. The encapsulated formulation of Btk IMBL-B9 presented an increased survival rate and storage stability at 54 ± 2°C for up to 6 weeks. The formulation showed similar insecticidal activity as the commercial bioinsecticide XenTari ® against P. xylostella . Under controlled greenhouse conditions, the Btk IMBL-B9 formulation was more effective against Lepidoptera spp. S. frugiperda and P. xylostella , than XenTari ® . These results suggest that the microencapsulation-based spray drying formulation of Btk IMBL-B9 can be used effectively for the control of a wide range of moths. Competing Interests: KD, NY, SK, JB, JS, EC, and J-CK were employed by Plant Healthcare Research Institute, JAN153 Biotech Incorporated. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Duraisamy, Yu, Kim, Baek, Son, Choi, Park, Kim, Choi, Sang, Je and Kim.)
Databáze:	MEDLINE
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