Identification of novel small RNAs in extracellular vesicles produced by Actinobacillus pleuropneumoniae .

Autor:	da Silva GC; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil., Rosa JN; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil., Fontes PP; Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., de Castro AG; Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Barbosa ÉAA; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Clarindo WR; Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Mantovani HC; Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Li Y; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom., Bossé JT; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom., Langford PR; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom., Bazzolli DMS; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Nov 23; Vol. 14, pp. 1291930. Date of Electronic Publication: 2023 Nov 23 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1291930
Abstrakt:	Extracellular vesicle (EV) production by bacteria is an important mechanism for microbial communication and host-pathogen interaction. EVs of some bacterial species have been reported to contain nucleic acids. However, the role of small RNAs (sRNAs) packaged in EVs is poorly understood. Here, we report on the RNA cargo of EVs produced by the pig pathogen Actinobacillus pleuropneumoniae , the causal agent of porcine pleuropneumonia, a disease which causes substantial economic losses to the swine industry worldwide. The EVs produced by aerobically and anaerobically grown bacteria were only slightly different in size and distribution. Total cell and outer membrane protein profiles and lipid composition of A. pleuropneumoniae whole cell extracts and EVs were similar, although EVs contained rough lipopolysaccharide compared to the smooth form in whole cells. Approximately 50% of Galleria mellonella larvae died after the injection of EVs. RNAseq, RT-PCR, protection from nuclease degradation, and database searching identified previously described and 13 novel A. pleuropneumoniae sRNAs in EVs, some of which were enriched compared to whole cell content. We conclude that A. pleuropneumoniae EVs contain sRNAs, including those known to be involved in virulence, and some with homologs in other Pasteurellaceae and/or non- Pasteurellaceae . Further work will establish whether the novel sRNAs in A. pleuropneumoniae EVs play any role in pathogenesis. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 da Silva, Rosa, Fontes, de Castro, Barbosa, Clarindo, Mantovani, Li, Bossé, Langford and Bazzolli.)
Databáze:	MEDLINE
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