Genome of Linum usitatissimum convar. crepitans expands the view on the section Linum .

Autor: Dvorianinova EM; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Pushkova EN; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Bolsheva NL; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Borkhert EV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Rozhmina TA; Federal Research Center for Bast Fiber Crops, Torzhok, Russia., Zhernova DA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia., Novakovskiy RO; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Turba AA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Sigova EA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.; Moscow Institute of Physics and Technology, Moscow, Russia., Melnikova NV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Dmitriev AA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: Frontiers in genetics [Front Genet] 2023 Nov 22; Vol. 14, pp. 1269837. Date of Electronic Publication: 2023 Nov 22 (Print Publication: 2023).
DOI: 10.3389/fgene.2023.1269837
Abstrakt: Sequencing whole plant genomes provides a solid foundation for applied and basic studies. Genome sequences of agricultural plants attract special attention, as they reveal information on the regulation of beneficial plant traits. Flax is a valuable crop cultivated for oil and fiber. Genome sequences of its representatives are rich sources of genetic information for the improvement of cultivated forms of the plant. In our work, we sequenced the first genome of flax with the dehiscence of capsules- Linum usitatissimum convar. сrepitans (Boenn.) Dumort-on the Oxford Nanopore Technologies (ONT) and Illumina platforms. We obtained 23 Gb of raw ONT data and 89 M of 150 + 150 paired-end Illumina reads and tested different tools for genome assembly and polishing. The genome assembly produced according to the Canu-Racon ×2-medaka-POLCA scheme had optimal contiguity and completeness: assembly length-412.6 Mb, N50-5.2 Mb, L50-28, and complete BUSCO-94.6% (64.0% duplicated, eudicots_odb10). The obtained high-quality genome assembly of L. usitatissimum convar. crepitans provides opportunities for further studies of evolution, domestication, and genome regulation in the section Linum .
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Dvorianinova, Pushkova, Bolsheva, Borkhert, Rozhmina, Zhernova, Novakovskiy, Turba, Sigova, Melnikova and Dmitriev.)
Databáze: MEDLINE