Enhancing bone tissue engineering with 3D-Printed polycaprolactone scaffolds integrated with tragacanth gum/bioactive glass.

Autor:	Janmohammadi M; Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran., Nourbakhsh MS; Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, Iran., Bahraminasab M; Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.; Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, 3513138111, Iran., Tayebi L; Marquette University School of Dentistry, Milwaukee, WI, 53233, USA.
Jazyk:	angličtina
Zdroj:	Materials today. Bio [Mater Today Bio] 2023 Nov 20; Vol. 23, pp. 100872. Date of Electronic Publication: 2023 Nov 20 (Print Publication: 2023).
DOI:	10.1016/j.mtbio.2023.100872
Abstrakt:	Tissue-engineered bone substitutes, characterized by favorable physicochemical, mechanical, and biological properties, present a promising alternative for addressing bone defects. In this study, we employed an innovative 3D host-guest scaffold model, where the host component served as a mechanical support, while the guest component facilitated osteogenic effects. More specifically, we fabricated a triangular porous polycaprolactone framework (host) using advanced 3D printing techniques, and subsequently filled the framework's pores with tragacanth gum-45S5 bioactive glass as the guest component. Comprehensive assessments were conducted to evaluate the physical, mechanical, and biological properties of the designed scaffolds. Remarkably, successful integration of the guest component within the framework was achieved, resulting in enhanced bioactivity and increased strength. Our findings demonstrated that the scaffolds exhibited ion release (Si, Ca, and P), surface apatite formation, and biodegradation. Additionally, in vitro cell culture assays revealed that the scaffolds demonstrated significant improvements in cell viability, proliferation, and attachment. Significantly, the multi-compartment scaffolds exhibited remarkable osteogenic properties, indicated by a substantial increase in the expression of osteopontin, osteocalcin, and matrix deposition. Based on our results, the framework provided robust mechanical support during the new bone formation process, while the guest component matrix created a conducive micro-environment for cellular adhesion, osteogenic functionality, and matrix production. These multi-compartment scaffolds hold great potential as a viable alternative to autografts and offer promising clinical applications for bone defect repair in the future. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.)
Databáze:	MEDLINE
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