Ameliorative effects of allogeneic and xenogenic bone marrow-derived mesechymal stem cells on carbon tetrachloride-induced rat liver injury and cirrhosis via modulation of oxidative stress, apoptosis, inflammation, and Nrf2 expression.

Autor:	Abdel Fattah AA; Division of Cell Biology, Histology and Genetics, Department of Zoology, Faculty of Science, Beni-Suef University Beni-Suef 62511, Egypt., Abdul-Hamid M; Division of Cell Biology, Histology and Genetics, Department of Zoology, Faculty of Science, Beni-Suef University Beni-Suef 62511, Egypt., Almanaa TN; Department of Botany and Microbiology, College of Science, King Saud University Riyadh 11451, Saudi Arabia., Alhaber LA; Department of Botany and Microbiology, College of Science, King Saud University Riyadh 11451, Saudi Arabia., Abdel-Kawi SH; Department of Histology, Faculty of Medicine, Beni-Suef University Beni-Suef 62521, Egypt., Abdel Rahman FES; Department of Basic Sciences, Faculty of Oral and Dental Medicine, Nahda University Beni-Suef 62764, Egypt., Ahmed OM; Division of Physiology, Department of Zoology, Faculty of Science, Beni-Suef University Beni-Suef 62521, Egypt.
Jazyk:	angličtina
Zdroj:	American journal of translational research [Am J Transl Res] 2023 Nov 15; Vol. 15 (11), pp. 6381-6403. Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023).
Abstrakt:	Objectives: The aim of this study was to compare the effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) isolated from mice (xenogeneic) and rats (allogeneic) on liver injury induced by carbon tetrachloride (CCl4) as well as to explore the modulatory effects on of oxidative stress, apoptosis, inflammation, and Nrf2 expression. Methods: Male Wistar rats were intraperitoneally injected with CCl4 (0.5 mL/kg) twice a week for 8 weeks. The animals were intravenously infused with BM-MSCs isolated from male mice or rats (1 × 10 6 cells/rat/week) into the lateral tail vein for 4 weeks. Results: The treatment with BM-MSCs produced a significant increase in the diminished serum albumin level, a significant decrease in liver lipid peroxidation and an increase in glutathione content as well as SOD, GST, and GPx activities. Furthermore, BM-MSCs from both mice and rats produced a significant decrease in the elevated mRNA expression of liver CYP1A1, MMP-9, procollagen α1, TGF-β1, and increase in expression of lowered IL-4, IL-10, cluster CD-105, and Oct3/4. In liver of CCl4-injected rats, the lower protein expression of Nrf2 was upregulated and higher expressions of caspase-3, TNF-R1, NF-κB p65, TNF-α, p53, and COX-2 were downregulated by mice and rats' BM-MSCs. Histologically, BM-MSCs from both mice and rats successfully improved liver structural integrity and protected against liver injury. Conclusions: The rats-derived BM-MSCs were significantly more potent than mice-derived BM-MSCs. Mice BM-MSCs and rats' BM-MSCs acted to improve CCl4-impaired liver function, structural integrity, fibrosis and cirrhosis in male Wistar rats via the suppression of oxidative stress, inflammation, and apoptosis and the enhancement of the antioxidant defense system. Competing Interests: None. (AJTR Copyright © 2023.)
Databáze:	MEDLINE
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