Extracellular vesicles and co-isolated endogenous retroviruses from murine cancer cells differentially affect dendritic cells.

Autor:	Cocozza F; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France.; Université de Paris, Paris, France., Martin-Jaular L; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France.; Institut Curie Centre de Recherche, CurieCoreTech Extracellular Vesicles, Paris, France., Lippens L; Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, and Cancer Research Institute Ghent, Ghent, Belgium., Di Cicco A; Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, Laboratoire Physico-chimie Curie, Paris, France.; Institut Curie, PSL Research University, CNRS UMR144, Cell and Tissue Imaging Facility (PICT-IBiSA), Paris, France., Arribas YA; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France., Ansart N; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France., Dingli F; Institut Curie, PSL Research University, Centre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Paris, France., Richard M; Institut Curie, PSL Research University, Centre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Paris, France., Merle L; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France., Jouve San Roman M; CNRS UMR3215, Institut Curie, PSL Research University, Paris, France., Poullet P; Institut Curie, Bioinformatics core facility (CUBIC), INSERM U900, PSL Research University, Mines Paris Tech, Paris, France., Loew D; Institut Curie, PSL Research University, Centre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Paris, France., Lévy D; Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, Laboratoire Physico-chimie Curie, Paris, France.; Institut Curie, PSL Research University, CNRS UMR144, Cell and Tissue Imaging Facility (PICT-IBiSA), Paris, France., Hendrix A; Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, and Cancer Research Institute Ghent, Ghent, Belgium., Kassiotis G; Retroviral Immunology, The Francis Crick Institute and Department of Medicine, Faculty of Medicine, Imperial College, London, UK., Joliot A; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France., Tkach M; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France., Théry C; INSERM U932, Institut Curie Centre de Recherche, PSL Research University, Paris, France.; Institut Curie Centre de Recherche, CurieCoreTech Extracellular Vesicles, Paris, France.
Jazyk:	angličtina
Zdroj:	The EMBO journal [EMBO J] 2023 Dec 11; Vol. 42 (24), pp. e113590.
DOI:	10.15252/embj.2023113590
Abstrakt:	Cells secrete extracellular vesicles (EVs) and non-vesicular extracellular (nano)particles (NVEPs or ENPs) that may play a role in intercellular communication. Tumor-derived EVs have been proposed to induce immune priming of antigen presenting cells or to be immuno-suppressive agents. We suspect that such disparate functions are due to variable compositions in EV subtypes and ENPs. We aimed to characterize the array of secreted EVs and ENPs of murine tumor cell lines. Unexpectedly, we identified virus-like particles (VLPs) from endogenous murine leukemia virus in preparations of EVs produced by many tumor cells. We established a protocol to separate small EVs from VLPs and ENPs. We compared their protein composition and analyzed their functional interaction with target dendritic cells. ENPs were poorly captured and did not affect dendritic cells. Small EVs specifically induced dendritic cell death. A mixed large/dense EV/VLP preparation was most efficient to induce dendritic cell maturation and antigen presentation. Our results call for systematic re-evaluation of the respective proportions and functions of non-viral EVs and VLPs produced by murine tumors and their contribution to tumor progression. (© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
Databáze:	MEDLINE
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