Capillary electrophoresis with capacitively coupled contactless conductivity detection (C 4 D) for rapid and simple determination of lactate in sweat.

Autor: Rocha KN; Instituto de Química, Universidade Estadual de Campinas, UNICAMP, Campinas, São Paulo, Brazil., da Silva JAF; Instituto de Química, Universidade Estadual de Campinas, UNICAMP, Campinas, São Paulo, Brazil.; Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, São Paulo, Brazil., de Jesus DP; Instituto de Química, Universidade Estadual de Campinas, UNICAMP, Campinas, São Paulo, Brazil.; Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2024 Mar; Vol. 45 (5-6), pp. 392-399. Date of Electronic Publication: 2023 Dec 10.
DOI: 10.1002/elps.202300179
Abstrakt: An analytical method based on capillary electrophoresis (CE) using capacitively coupled contactless conductivity detection (C 4 D) was developed and validated for fast, straightforward, and reliable determination of lactate in artificial and human sweat samples. The background electrolyte was composed of equimolar concentrations (10 mmol/L) of 2-(N-morpholino)ethanesulfonic acid and histidine, with 0.2 mmol/L of cetyltrimethylammonium bromide as electroosmotic flow inverter. The limit of detection and quantification were 3.1 and 10.3 µmol/L, respectively. Recoveries in the 97 to 118% range were obtained using sweat samples spiked with lactate at three concentration levels, indicating an acceptable accuracy. The intraday and interday precisions were 1.49 and 7.08%, respectively. The proposed CE-C 4 D method can be a starting point for monitoring lactate concentrations in sweat samples for diagnostics, physiological studies, and sports performance assessment applications.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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