Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection.

Autor: Saffari Z; Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, 1316943551, Iran., Cohan RA; Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, 1316943551, Iran., Sepahi M; Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, 1316943551, Iran., Sadeqi M; Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran., Khoobi M; Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.; Pharmaceutical Quality Assurance Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran., Fard MH; Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran., Ghavidel A; Physics Department, Sharif University of Technology, Tehran, Iran., Amiri FB; Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran., Aghasadeghi MR; Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran. Mr_sadeqi@yahoo.Com., Norouzian D; Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, 1316943551, Iran. dnsa@pasteur.ac.ir.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Dec 09; Vol. 13 (1), pp. 21851. Date of Electronic Publication: 2023 Dec 09.
DOI: 10.1038/s41598-023-48766-2
Abstrakt: The procedures currently used for hepatitis B (HB) detection are not suitable for screening, clinical diagnosis, and point-of-care testing (POCT). Therefore, we developed and tested a QCM-based immunosensor by surface modification with AuNP-PEIs to amplify the signal and provide an oriented-immobilization surface. The AuNP-PEIs were characterized by ICP-Mass, UV/Vis, DLS, FE-SEM, and ATR-FTIR. After coating AuNP-PEIs on the gold electrode surface, anti-HBsAg antibodies were immobilized using NHS/EDC chemistry based on response surface methodology (RSM) optimization. The efficiency of the immunosensor was assessed by human sera and data were compared to gold-standard ELISA using receiver-operating-characteristic (ROC) analysis. FE-SEM, AFM, EDS, and EDS mapping confirmed AuNP-PEIs are homogeneously distributed on the surface with a high density and purity. After antibody immobilization, the immunosensor exhibited good recognition of HBsAg with a calibration curve of ∆F =  - 6.910e -7 x + 10(R 2  = 0.9905), a LOD of 1.49 ng/mL, and a LOQ of 4.52 ng/mL. The immunosensor yielded reliable and accurate results with a specificity of 100% (95% CI 47.8-100.0) and sensitivity of 100% (95% CI 96.2-100.0). In conclusion, the fabricated immunosensor has the potential as an analytic tool with high sensitivity and specificity. However, further investigations are needed to convert it to a tiny lab-on-chip for HB diagnosis in clinical samples.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje