Enhanced ethanol production from paper sludge waste under high-solids conditions with industrial and cellulase-producing strains of Saccharomyces cerevisiae.

Autor: van Dyk J; Dept. of Chemical Engineering, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address: 22215050@sun.ac.za., Görgens JF; Dept. of Chemical Engineering, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address: jgorgens@sun.ac.za., van Rensburg E; Dept. of Chemical Engineering, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address: eugenevrb@sun.ac.za.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Feb; Vol. 394, pp. 130163. Date of Electronic Publication: 2023 Dec 07.
DOI: 10.1016/j.biortech.2023.130163
Abstrakt: Reported ethanol titres from hydrolysis-fermentation of the degraded fibres in paper sludge (PS) waste, generally obtained under fed-batch submerged conditions, can be improved through fermentation processes at high solids loadings, as demonstrated in the present study with two industrial PS wastes at enzyme dosages appropriate for solids loadings up to 40% (w/w). The industrial yeast,Saccharomyces cerevisiaestrain Ethanol Red®, was compared to two genetically engineeredS. cerevisiaestrains, namely Cellusec® 1.0 and Cellusec® 2.0, capable of xylose utilisation, and xylose utilisation and cellulase production, respectively. High-solids batch fermentations were conducted in 3 L horizontal rotating reactors and ethanol titres of 100.8 and 73.3 g/L were obtained for virgin pulp and corrugated recycle PS, respectively, at 40% (w/w) solids loading using Ethanol Red®. Xylose utilisation by Cellusec® 1.0 improved ethanol titres by up to 10.3%, while exogenous cellulolytic enzyme requirements were reduced by up to 50% using cellulase-producing Cellusec® 2.0.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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