Production of highly antioxidant lignin nanoparticles from a hardwood technical lignin.

Autor: Pavaneli G; Department of Chemistry, Federal University of Paraná, P.O. Box 19032, Curitiba, PR 81531-980, Brazil., da Silva TA; Department of Chemistry, Federal University of Paraná, P.O. Box 19032, Curitiba, PR 81531-980, Brazil., Zawadzki SF; Department of Chemistry, Federal University of Paraná, P.O. Box 19032, Curitiba, PR 81531-980, Brazil., Sassaki GL; Department of Biochemistry and Molecular Biology, Federal University of Paraná, P.O. Box 19046, Curitiba, PR 81531-980, Brazil., de Freitas RA; Department of Pharmacy, Federal University of Paraná, P.O. Box 19005, Curitiba, PR 80210-170, Brazil., Ramos LP; Department of Chemistry, Federal University of Paraná, P.O. Box 19032, Curitiba, PR 81531-980, Brazil. Electronic address: luiz.ramos@ufpr.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Feb; Vol. 257 (Pt 1), pp. 128612. Date of Electronic Publication: 2023 Dec 07.
DOI: 10.1016/j.ijbiomac.2023.128612
Abstrakt: Eucalypt kraft lignin isolated in a LignoBoost™ pilot plant was characterized by GC-MS, ICP-OES, DSC, HPSEC, 31 P NMR, and HSQC 2D-NMR to be used without any further processing to produce lignin nanoparticles (LNPs) by nanoprecipitation. Tetrahydrofuran (THF) was used as a solvent, and water as a non-solvent. Microscopic analysis (TEM) showed that LNPs were regularly spherical with some hollow particles dispersed in-between, and sizes were tunable by changing the solvent dripping rate onto the non-solvent. LNP particle sizes had a bimodal distribution, with the largest population having an average apparent hydrodynamic diameter ranging from 105.6 to 75.6 nm. Colloidal dispersions of LNPs in water presented good stability in different dilutions without significant size changes upon storage at pH close to neutral for as long as 45 days. Zeta potentials around -40 mV were obtained for LNP suspensions at pH ranging from 7 to 9. The high carbohydrate content (circa 10 % on a dry basis, mostly xylans) of the lignin precursor did not interfere in LNP formation, whose antioxidant activity was expressive as demonstrated by the ABTS assay at pH 7.4, with an EC 50 of 4.04 μg mL -1 . Also, the Trolox® equivalent antioxidant capacity (TEAC) of LNPs reached 1.90 after 40 min reaction time.
Competing Interests: Declaration of competing interest Luiz Pereira Ramos reports financial support was provided by National Council for Scientific and Technological Development. Giuliana Pavaneli reports financial support was provided by Coordination of Higher Education Personnel Improvement. Nothing to declare.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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