| Autor: |
Semenescu AD; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Moacă EA; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Iftode A; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Dehelean CA; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Tchiakpe-Antal DS; Department of Pharmaceutical Botany, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Vlase L; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, 'Iuliu Hatieganu' University of Medicine and Pharmacy, 8th Victor Babes Street, 400347 Cluj-Napoca, Romania., Vlase AM; Department of Pharmaceutical Botany, Faculty of Pharmacy, 'Iuliu Hatieganu' University of Medicine and Pharmacy, 8th Victor Babes Street, 400347 Cluj-Napoca, Romania., Muntean D; Department of Microbiology, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Multidisciplinary Research Center on Antimicrobial Resistance, 'Victor Babes' University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Chioibaş R; Department of Surgery I, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timișoara, Romania.; CBS Medcom Hospital, 12th Popa Sapca Street, 300047 Timisoara, Romania. |
| Abstrakt: |
Galium species are used worldwide for their antioxidant, antibacterial, antifungal, and antiparasitic properties. Although this plant has demonstrated its antitumor properties on various types of cancer, its biological activity on cutaneous melanoma has not been established so far. Therefore, the present study was designed to investigate the phytochemical profile of two extracts of G. verum L. herba (ethanolic and ethyl acetate) as well as the biological profile (antioxidant, antimicrobial, and antitumor effects) on human skin cancer. The extracts showed similar FT-IR phenolic profiles (high chlorogenic acid, isoquercitrin, quercitrin, and rutin), with high antioxidant capacity (EC 50 of ethyl acetate phase (0.074 ± 0.01 mg/mL) > ethanol phase (0.136 ± 0.03 mg/mL)). Both extracts showed antimicrobial activity, especially against Gram-positive Streptococcus pyogenes and Staphylococcus aureus bacilli strains, the ethyl acetate phase being more active. Regarding the in vitro antitumor test, the results revealed a dose-dependent cytotoxic effect against A375 melanoma cell lines, more pronounced in the case of the ethyl acetate phase. In addition, the ethyl acetate phase stimulated the proliferation of human keratinocytes (HaCaT), while this effect was not evident in the case of the ethanolic phase at 24 h post-stimulation. Consequently, G. verum l. could be considered a promising phytocompound for the antitumor approach of cutaneous melanoma. |
