Carnosine supplementation and retinal oxidative parameters in a high-calorie diet rat model.

Autor: de Almeida Torres RJ; Medical School, Department of Internal Medicine, Universidade Estadual Paulista (UNESP), Botucatu, SP, 18618-687, Brazil. rjat@terra.com.br., Moreto F; Medical School, Department of Internal Medicine, Universidade Estadual Paulista (UNESP), Botucatu, SP, 18618-687, Brazil., Luchini A; Department of Ophthalmology, Centro Oftalmologico de Curitiba, Curitiba, PR, Brazil., de Almeida Torres RJ; Department of Ophthalmology, Hospital Angelina Caron, Campina Grande Do Sul, PR, Brazil., Longo SP; Postgraduate Program in Health Sciences, School of Medicine, Pontificia Universidade Catolica Do Paraná, Curitiba, PR, Brazil., Pinho RA; Postgraduate Program in Health Sciences, School of Medicine, Pontificia Universidade Catolica Do Paraná, Curitiba, PR, Brazil., Nagashima S; Postgraduate Program in Health Sciences, School of Medicine, Pontificia Universidade Catolica Do Paraná, Curitiba, PR, Brazil., de Noronha L; Postgraduate Program in Health Sciences, School of Medicine, Pontificia Universidade Catolica Do Paraná, Curitiba, PR, Brazil., Ferron AJT; Medical School, Department of Internal Medicine, Universidade Estadual Paulista (UNESP), Botucatu, SP, 18618-687, Brazil., de Almeida Silva CCV; Medical School, Department of Internal Medicine, Universidade Estadual Paulista (UNESP), Botucatu, SP, 18618-687, Brazil., Correa CR; Medical School, Department of Internal Medicine, Universidade Estadual Paulista (UNESP), Botucatu, SP, 18618-687, Brazil., Aldini G; Dipartimento Di Scienze Farmaceutiche (DISFARM), Università Degli Studi Di Milano, Milan, Italy., Ferreira ALA; Medical School, Department of Internal Medicine, Universidade Estadual Paulista (UNESP), Botucatu, SP, 18618-687, Brazil.
Jazyk: angličtina
Zdroj: BMC ophthalmology [BMC Ophthalmol] 2023 Dec 08; Vol. 23 (1), pp. 502. Date of Electronic Publication: 2023 Dec 08.
DOI: 10.1186/s12886-023-03255-y
Abstrakt: Background: To assess oxidative effects induced by a high-calorie diet on the retina of Wistar rats and test the antioxidative effects of carnosine supplementation.
Methods: Wistar rats were randomly divided into the following groups: standard diet (SD), high-calorie diet (HcD), standard diet + carnosine (SD + Car), and high-calorie diet + carnosine (HcD + Car). The body weight, adiposity index, plasma glucose, total lipids, high-density lipoprotein (HDL), low-density lipoprotein (LDL), uric acid, creatinine, and triglycerides of the animals were evaluated. The retinas were analyzed for markers of oxidative stress. Hydrogen peroxide production was assessed by 2',7'-dichlorodihydrofluorescein diacetate (DCF) oxidation. The total glutathione (tGSH), total antioxidant capacity (TAC), protein carbonyl, and sulfhydryl groups of the antioxidant system were analyzed.
Results: TAC levels increased in the retinas of the SD + Car group compared to the SD group (p < 0.05) and in the HcD + Car group compared to the HcD group (p < 0.05). The levels of GSH and the GSSH:GSSG ratio were increased in the HcD + Car group compared to the SD + Car group (p < 0.05). An increase in the retinal carbonyl content was observed in the HcD group compared to the SD group (p < 0.05) and in the HcD + Car group compared to the SD + Car group (p < 0.05). A high-calorie diet (HcD) was also associated with a decrease in retinal sulfhydryl-type levels compared to the SD group (p < 0.05).
Conclusion: The results suggest that feeding a high-calorie diet to rats can promote an increase in carbonyl content and a reduction in sulfhydryl groups in their retinas. The administration of carnosine was not effective in attenuating these oxidative markers.
Trial Registration: Animal Ethics Committee of Botucatu Medical School - Certificate number 1292/2019.
(© 2023. The Author(s).)
Databáze: MEDLINE
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