Anti-Bordetella bronchiseptica effects of targeted bacteriophages via microbiome and metabolic mediated mechanisms.

Autor: Hosseindoust A; Department of Animal Industry Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea., Choi Y; Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 31000, Republic of Korea., Ha S; Department of Animal Industry Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea., Tajudeen H; Department of Animal Industry Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea., Mun J; Department of Animal Industry Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea., Kinara E; Department of Animal Industry Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea., Kim Y; CTC Bio, Inc., Seoul, 138-858, Republic of Korea., Kim J; Department of Animal Industry Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea. kjs896@kangwon.ac.kr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Dec 08; Vol. 13 (1), pp. 21755. Date of Electronic Publication: 2023 Dec 08.
DOI: 10.1038/s41598-023-49248-1
Abstrakt: Bordetella bronchiseptica poses a significant challenge in the context of respiratory infections, particularly in weanling pigs. In this study, we investigated the impact of a novel targeted bacteriophage in controlling B. bronchiseptica challenge (BBC) in an experimental design involving five distinct treatment groups: NC (no challenge), PC (BBC challenge), BF (10 8 pfu bacteriophage/kg diet + BBC), BN (2 × 10 7 pfu/day bacteriophage by nasal spray + BBC), and AT (antibiotic + BBC). The experiment was conducted for 2 weeks. The highest turbinate score was observed in the PC. The BF treatment showed higher plasma IL (interleukine)-1β and IL-6 compared with the BN and AT treatments. Plasma concentrations of IL-1β were increased in the BF pigs compared with the BN, AT, and NC. Among the BBC groups, the PC treatment exhibited a higher abundance of Staphylococcus. aureus and B. bronchiseptica in the lung. A lower S. aureus, Streptococcus. suis, and B. bronchiseptica colonization was detected in the AT compared with the BF and BN treatments. The BF showed lower plasma zonulin compared with the BN and AT. A higher plasma concentration of superoxide dismutase was observed in the BF and AT compared with PC and BN. The BN influenced the glycine, serine-threonine metabolism; glycerolipid metabolism; glyoxylate-dicarboxylate metabolism; and arachidonic acid metabolism compared with the NC. In conclusion, nasal-sprayed bacteriophage effectively controlled B. bronchiseptica infection, however, their efficiency was lower than the antibiotic.
(© 2023. The Author(s).)
Databáze: MEDLINE
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