Tear Fluid Inflammatory Proteome Analysis Highlights Similarities Between Keratoconus and Allergic Conjunctivitis.

Autor:	Gijs M; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands., Adelaar TI; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands., Vergouwen DPC; Department of Ophthalmology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.; Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands., Visser N; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands., Dickman MM; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands., Ollivier RCI; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands., Berendschot TTJM; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands., Nuijts RMMA; University Eye Clinic Maastricht, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, The Netherlands.; Department of Ophthalmology, Zuyderland Medical Center, Heerlen, The Netherlands.
Jazyk:	angličtina
Zdroj:	Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2023 Dec 01; Vol. 64 (15), pp. 9.
DOI:	10.1167/iovs.64.15.9
Abstrakt:	Purpose: Keratoconus is characterized by the progressive thinning of the cornea, which leads to a cone-like appearance of the eye over time. Although conventionally defined as a noninflammatory condition, a number of recent studies have associated keratoconus (KC) with allergic conjunctivitis (AC) based on clinical parameters. This study aimed to consolidate this association by performing a proteomic analysis of tear fluid from patients with keratoconus and/or allergic conjunctivitis. Methods: Of 51 patients, 17 were diagnosed with KC, 17 were diagnosed with AC, and 17 were diagnosed with both KC and AC (combined). Nine of 34 patients with KC had a progressive form of the disease. Tear fluid samples (n = 51, one eye per patient) were collected by the Schirmer's strips. Tear proteins were extracted from the Schirmer's strips. Proteomic profiling of 384 inflammatory proteins was assessed by a multiplex proximity extension assay (Olink Explore 384 Inflammation Panel I). Results: A total of 384 inflammatory proteins were measured. Two hundred seventy-two of the 384 proteins passed stringent data cleaning and were compared among the patient groups. Compared to the 2 other groups, LGALS9 was upregulated uniquely in KC, whereas FGF19, PDGFB, HPCAL1, OSM, and FCAR were downregulated in KC. Similarly, TNFRSF4 and CCL13 were specifically upregulated in AC, whereas ectodysplasin A receptor (EDAR) was uniquely downregulated in AC. Conclusions: High-throughput proteomic profiling of tear fluid confirms the association between KC and AC on a molecular level and raise the importance of redefining KC as an inflammatory condition.
Databáze:	MEDLINE
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