| Autor: |
Dooijewaard G, Roossien FF, Robillard GT |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry [Biochemistry] 1979 Jul 10; Vol. 18 (14), pp. 2990-6. |
| DOI: |
10.1021/bi00581a013 |
| Abstrakt: |
A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state kinetic measurements of the phosphotransferase activity demonstrate that the polysaccharide works as an activator of the phosphotransferase system probably at the level of the HPr:P-E1 complex or the P-HPr:E11 complex. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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