Ensuring quality in 17OHP mass spectrometry measurement: an international study assessing isomeric steroid interference.

Autor:	Ho CS; Department of Chemical Pathology, Biomedical Mass Spectrometry Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR China., Hoad K; Department of Clinical Biochemistry, PathWest Laboratory Medicine, Fiona Stanley Hospital, Perth, WA, Australia., Cooke BR; Department of Clinical Biochemistry, PathWest Laboratory Medicine, Fiona Stanley Hospital, Perth, WA, Australia., Andersen T; Australian Scientific Enterprise, Hornsby, NSW, Australia., Graham P; Royal College of Pathologists of Australasia Quality Assurance Programs, Sydney, NSW, Australia., van den Berg SAA; Department Internal Medicine, Erasmus MC, University Medical Centre, Rotterdam, The Netherlands.; Department Clinical Chemistry, Erasmus MC, University Medical Centre, Rotterdam, The Netherlands., Hartmann MF; Steroid Research & Mass Spectrometry Unit of the Laboratory for Translational Hormone Analytics in Pediatric Endocrinology at the Justus Liebig University in Giessen, Giessen, Germany., Lo CWS; Department of Chemical Pathology, Biomedical Mass Spectrometry Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR China., Loh TP; Department of Laboratory Medicine, National University Hospital, Singapore, Singapore., de Rijke YB; Department Clinical Chemistry, Erasmus MC, University Medical Centre, Rotterdam, The Netherlands., van Zelst BD; Department Internal Medicine, Erasmus MC, University Medical Centre, Rotterdam, The Netherlands., Wudy SA; Steroid Research & Mass Spectrometry Unit of the Laboratory for Translational Hormone Analytics in Pediatric Endocrinology at the Justus Liebig University in Giessen, Giessen, Germany., Zakaria R; School of Health and Biomedical Sciences, RMIT University, Melbourne, VIC, Australia.; Murdoch Children's Research Institute, Parkville, VIC, Australia., Greaves RF; Victorian Clinical Genetics Services, Murdoch Children's Research Institute, The Royal Children's Hospital Melbourne, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.
Jazyk:	angličtina
Zdroj:	Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2023 Dec 11; Vol. 62 (5), pp. 911-918. Date of Electronic Publication: 2023 Dec 11 (Print Publication: 2024).
DOI:	10.1515/cclm-2023-0864
Abstrakt:	Objectives: Interference from isomeric steroids is a potential cause of disparity between mass spectrometry-based 17-hydroxyprogesterone (17OHP) results. We aimed to assess the proficiency of mass spectrometry laboratories to report 17OHP in the presence of known isomeric steroids. Methods: A series of five samples were prepared using a previously demonstrated commutable approach. These samples included a control (spiked to 15.0 nmol/L 17OHP) and four challenge samples further enriched with equimolar concentrations of 17OHP isomers (11α-hydroxyprogesterone, 11β-hydroxyprogesterone, 16α-hydroxyprogesterone or 21-hydroxyprogesterone). These samples were distributed to 38 participating laboratories that reported serum 17OHP results using mass spectrometry in two external quality assurance programs. The result for each challenge sample was compared to the control sample submitted by each participant. Results: Twenty-six laboratories (68 % of distribution) across three continents returned results. Twenty-five laboratories used liquid chromatography-tandem mass spectrometry (LC-MS/MS), and one used gas chromatography-tandem mass spectrometry to measure 17OHP. The all-method median of the control sample was 14.3 nmol/L, ranging from 12.4 to 17.6 nmol/L. One laboratory had results that approached the lower limit of tolerance (minus 17.7 % of the control sample), suggesting the isomeric steroid caused an irregular result. Conclusions: Most participating laboratories demonstrated their ability to reliably measure 17OHP in the presence of the four clinically relevant isomeric steroids. The performance of the 12 (32 %) laboratories that did not engage in this activity remains unclear. We recommend that all laboratories offering LC-MS/MS analysis of 17OHP in serum, plasma, or dried bloodspots determine that the isomeric steroids are appropriately separated. (© 2023 the author(s), published by De Gruyter, Berlin/Boston.)
Databáze:	MEDLINE
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