Inhibition of biofilm, quorum-sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles.

Autor: Doğaç Yİ; Department of Chemistry and Chemical Processing Technology, Mugla Vocational School, Mugla Sitki Kocman University, Mugla, Turkey., Tamfu AN; Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere, Cameroon.; Scientific Analysis Technological Application and Research Center (UBATAM), Usak University, Usak, Turkey.; Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Muğla Sitki Koçman University, Ula, , Muğla, Turkey., Bozkurt S; Scientific Analysis Technological Application and Research Center (UBATAM), Usak University, Usak, Turkey.; Vocational School of Health Services, Usak University, Usak, Turkey., Kayhan M; Scientific Analysis Technological Application and Research Center (UBATAM), Usak University, Usak, Turkey., Teke M; Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey., Ceylan O; Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Muğla Sitki Koçman University, Ula, , Muğla, Turkey.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2024 Apr; Vol. 71 (2), pp. 356-371. Date of Electronic Publication: 2023 Dec 07.
DOI: 10.1002/bab.2545
Abstrakt: Resistance to antibiotics by pathogenic bacteria constitutes a health burden and nanoparticles (NPs) are being developed as alternative and multipurpose antimicrobial substances. Magnetite (Fe 3 O 4 np), manganese ferrite (MnFe 2 O 4 np) and nickel ferrite (NiFe 3 O 4 np) NPs were synthesized and characterized using thermogravimetric analysis, transmission electron microscopy, Fourier transformed infra-red, and X-ray diffraction. The minimal inhibitory concentrations (MIC) ranged from 0.625 to 10 mg/mL against gram-positive (Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), gram-negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) and candida (Candida albicans ATCC 10239 and Candida tropicalis ATCC 13803) species. The NPs exhibited violacein inhibition against Chromobacterium violaceum CV12472 of 100% at MIC and reduced to 27.2% ± 0.8% for magnetite NPs, 12.7% ± 0.3% for manganese ferrite NPs and 43.1% ± 0.2% for nickel ferrite NPs at MIC/4. Quorum-sensing (QS) inhibition zones against C. violaceum CV026 were 12.5 ±0.6 mm for Fe 3 O 4 np, 09.1 ± 0.5 mm for MnFe 3 O 4 NP and 17.0 ± 1.2 mm for NiFe 3 O 4 np. The NPs inhibited swarming motility against P. aeruginosa PA01 and biofilm against six pathogens and the gram-positive biofilms were more susceptible than the gram-negative ones. The NiFe 2 O 4 np had highest antibiofilm activity against gram-positive and gram-negative bacteria as well as highest QS inhibition while Fe 3 O 4 NP had highest biofilm inhibition against candida species. The synthesized magnetic NPs can be used in developing anti-virulence drugs which reduce pathogenicity of bacteria as well as resistance.
(© 2023 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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