Isolation and identification of vapA-absent Aeromonas salmonicida in diseased snakehead Channa argus in China.

Autor: Sun XN; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Wang Q; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Wang YF; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Tao Y; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Zheng CL; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Wang MH; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Che MY; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Cui ZH; School of Marine Sciences, Ningbo University, 315832, Ningbo, Zhejiang, China., Li XL; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Zhang Q; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Xu MX; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Wang S; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Nie P; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China., Sun YL; School of Marine Science and Engineering, Qingdao Agricultural University, 266237, Qingdao, Shandong, China. 201901116@qau.edu.cn.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Aug; Vol. 27 (4), pp. 1137-1150. Date of Electronic Publication: 2023 Dec 08.
DOI: 10.1007/s10123-023-00455-w
Abstrakt: Aeromonas salmonicida is the typical pathogen causing furunculosis, reported widely in salmonids. Because of multiple serotypes, the control of A. salmonicida-caused disease has increasingly received much attention. Recently, A. salmonicida infection was reported in non-salmonid fish species. Here, a pathogenic A. salmonicida, named as As-s, was isolated from cultured snakehead (Channa argus) in a local fish farm in Shandong, China. As-s displayed clear hemolysis, amylase, and positive catalase activities, and grew at a wide range of temperatures (10-37 °C) and pH values (5.5-8.5). As-s was highly sensitive to cefuroxime sodium, ceftriaxone, ceftazidime, piperacillin, and cefoperazone and also apparently sensitive to chloramphenicol, erythromycin, and 25% cinnamaldehyde. The Virulence array protein gene cloning' results suggested that As-s has this gene compared with the other two vapA-containing strains, despite a close relationship of these strains via phylogenetic analysis. Severe ulcers on skin, muscle, and abnormal liver, and hemorrhage in pectoral/ventral fins and anal region were observed, and exophthalmos were also noticed in infected juvenile snakehead, as well as necrosis and infiltration of blood cells emerged in the internal organs using pathological section. In addition, As-s caused high mortality in snakehead, consistently with its immune gene response. This study reports the first isolation of vapA-absent A. salmonicida in snakehead.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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