Electroacupuncture attenuates inflammatory pain via peripheral cannabinoid receptor type 1 signaling pathway in mice.

Autor: Ho TJ; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; School of Post‑Baccalaureate Chinese Medicine, Tzu Chi University, Hualien, Taiwan., Lin CF; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan., Chen JK; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; School of Post‑Baccalaureate Chinese Medicine, Tzu Chi University, Hualien, Taiwan., Kung YL; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan., Wu LK; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan., Chang Chien CY; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan., Huang CP; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.; Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Dec 07; Vol. 18 (12), pp. e0295432. Date of Electronic Publication: 2023 Dec 07 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0295432
Abstrakt: Pain is strongly associated with neuro-immune activation. Thus, the emerging role of the endocannabinoid system in neuro-inflammation is important. Acupuncture has been used for over 2500 years and is widely accepted for the management of pain. Our study aimed to investigate the effects of electroacupuncture on the regulation of cannabinoid receptor type 1 within the peripheral nervous system. Inflammatory pain was induced by injecting Complete Freund's adjuvant to induce mechanical and thermal hyperalgesia. Electroacupuncture significantly attenuated the mechanical and thermal sensitivities, and AM251, a cannabinoid receptor type 1 antagonist, eliminated these effects. Dual immunofluorescence staining demonstrated that electroacupuncture elevated expression of cannabinoid receptor type 1, co-localized with Nav 1.8. Furthermore, electroacupuncture significantly reduced levels of Nav 1.8 and COX-2 by western blot analysis, but not vice versa as AM251 treatment. Our data indicate that electroacupuncture mediates antinociceptive effects through peripheral endocannabinoid system signaling pathway and provide evidence that electroacupuncture is beneficial for pain treatment.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Ho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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