Parasitological and molecular characterization of Trypanosoma cruzi of triatomines and mammals from endemic areas for Chagas disease in Venezuela.

Autor: Morocoima A; Centro de Medicina Tropical de Oriente, Universidad de Oriente (UDO), Núcleo Anzoátegui, Barcelona, Estado Anzoátegui, Venezuela., Herrera L; Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela.; Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de La Salud, Universidad Nacional de Asunción, Central, Asunción, Paraguay., Rattia C; Centro de Medicina Tropical de Oriente, Universidad de Oriente (UDO), Núcleo Anzoátegui, Barcelona, Estado Anzoátegui, Venezuela., Figueroa M; Centro de Medicina Tropical de Oriente, Universidad de Oriente (UDO), Núcleo Anzoátegui, Barcelona, Estado Anzoátegui, Venezuela., Ferrer E; Instituto de Investigaciones Biomédicas 'Dr, Francisco J. Triana Alonso' (BIOMED), Universidad de Carabobo Sede Aragua, Calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, Estado Aragua, Venezuela. elizabeth.ferrer@gmail.com.; Departamento de Parasitología, Facultad de Ciencias de La Salud, Universidad de Carabobo Sede Aragua, Maracay, Estado Aragua, Venezuela. elizabeth.ferrer@gmail.com.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2023 Dec 07; Vol. 123 (1), pp. 17. Date of Electronic Publication: 2023 Dec 07.
DOI: 10.1007/s00436-023-08072-2
Abstrakt: It is estimated that 6-7 million people worldwide are infected with Trypanosoma cruzi, the parasite that causes Chagas disease. In Venezuela, Chagas disease remains a public health problem. In this work, T. cruzi isolates from six species of triatomines and mammals of the orders Didelphimorphia and Xenarthra, captured in rural communities of Monagas, underwent parasitological and molecular characterization. A total of 471 triatomines and 17 mammals were captured, with a natural infection rate of 41.4% and 70.6%, respectively. In the male NMRI mouse model used for parasitological characterization (prepatent period, parasitemia curve, mouse mortality, and tissular parasitism), T. cruzi isolates exhibited high lethality due to their pronounced virulence, irrespective of the parasite load in each mouse, resulting in a mortality rate of 75%. Among the vector isolates, in the mouse model, only 2 out of 6 remained alive, while the rest perished during the evaluation. Conversely, the isolates from mammals proved fatal for all the inoculated mice. All isolates were identified as belonging to DTU TcI, based on the molecular markers such as the intergenic region of the miniexon, D7 divergent domain of the 24Sα rDNA, size-variable domain of the 18S rDNA, and hsp60-PCR-RFLP-EcoRV. This study demonstrates the presence of vectors and mammalian reservoirs naturally infected with T. cruzi in communities of Monagas, the 9th largest and 9th most populous state in Venezuela. This situation represents a neglected epidemiological problem demanding urgent attention and imperative health care intervention.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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