Eligibility and efficacy of a CPC- and CHX-based antiviral mouthwash for the elimination of SARS-CoV-2 from the saliva: A randomized, double-blind, controlled clinical trial.

Autor: Brunello G; Department of Oral Surgery, University Hospital Düsseldorf, Düsseldorf, Germany.; Department of Neurosciences, Dentistry Section, University of Padua, Padua, Italy., Wolf V; Department of Oral Surgery, University Hospital Düsseldorf, Düsseldorf, Germany., Kerberger R; Department of Orthodontics and Dentofacial Orthopaedics, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Department of Orthodontics, University Hospital Düsseldorf, Düsseldorf, Germany., Bernhard M; Emergency Department, University Hospital Düsseldorf, Düsseldorf, Germany., Lübke N; Institute of Virology, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany., Becker J; Department of Oral Surgery, University Hospital Düsseldorf, Düsseldorf, Germany., Schwarz-Herzke B; Institute for Anatomy II, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany., Timm J; Institute of Virology, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany., Becker K; Department of Orthodontics and Dentofacial Orthopaedics, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Jazyk: angličtina
Zdroj: Journal of clinical periodontology [J Clin Periodontol] 2024 Feb; Vol. 51 (2), pp. 158-166. Date of Electronic Publication: 2023 Dec 07.
DOI: 10.1111/jcpe.13905
Abstrakt: Aim: This study aimed at investigating the efficacy of a 0.05% cetylpyridinium chloride-0.05% chlorhexidine (CPC-CHX) mouthwash in reducing viral load in the saliva as compared with sterile water.
Materials and Methods: Forty SARS-CoV-2 positive patients were asked to dispense 4 mL of saliva. Half the patients rinsed for 60 s with 15 mL CPC-CHX, and the remaining patients rinsed with sterile water (control). Four millilitres of saliva were collected after 15, 30 and 60 min after rinsing. Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) specific for SARS-CoV-2 nucleocapsid protein were performed. For ELISA, the intact (representing the active virus) to total virus load (I/T) was calculated.
Results: SARS-CoV-2 copy numbers/mL from RT-qPCR tended to decrease in the control group, whereas in the CPC-CHX group, an increase was observed after T30. However, mixed linear model analysis revealed no statistical differences between groups (p = .124), time points (p = .616) and vaccinated or non-vaccinated patients (p = .953). Similarly, no impact of group (p = .880), time points (p = .306) and vaccination (p = .711) was observed for I/T ratio values.
Conclusions: Within the limitation of this study, there was no evidence that the intervention reduced salivary SARS-CoV-2 viral load during the course of 60 min. Therefore, commonly used pre-procedural rinsing might not be clinically relevant.
(© 2023 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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