Molecular and functional characterization of chemosensory genes from the root-knot nematode Meloidogyne graminicola.

Autor: Dutta TK; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. tushar.dutta@icar.gov.in., Akhil VS; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Dash M; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Kundu A; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Phani V; Department of Agricultural Entomology, College of Agriculture, Uttar Banga Krishi Viswavidyalaya, Balurghat, Dakshin Dinajpur, West Bengal, India., Sirohi A; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2023 Dec 06; Vol. 24 (1), pp. 745. Date of Electronic Publication: 2023 Dec 06.
DOI: 10.1186/s12864-023-09864-7
Abstrakt: Background: Root-knot nematode Meloidogyne graminicola has emerged as a major threat in rice agroecosystems owing to climate change-induced changes in cultivation practices. Synthetic nematicides are continually being withdrawn from the nematode management toolbox because of their ill effects on the environment. A sustainable strategy would be to develop novel nematicides or resistant plants that would target nematode sensory perception, which is a key step in the host finding biology of plant-parasitic nematodes (PPNs). However, compared to the extensive literature on the free-living nematode Caenorhabditis elegans, negligible research has been performed on PPN chemosensory biology.
Results: The present study characterizes the five chemosensory genes (Mg-odr-7, Mg-tax-4, Mg-tax-4.1, Mg-osm-9, and Mg-ocr-2) from M. graminicola that are putatively associated with nematode host-finding biology. All the genes were highly transcribed in the early life stages, and RNA interference (RNAi)-induced downregulation of each candidate gene perturbed the normal behavioural phenotypes of M. graminicola, as determined by examining the tracking pattern of juveniles on Pluronic gel medium, attraction to and penetration in rice root tip, and developmental progression in rice root. In addition, a detrimental effect on nematode chemotaxis towards different volatile and nonvolatile organic compounds and host root exudates was documented.
Conclusion: Our findings enrich the existing literature on PPN chemosensory biology and can supplement future research aimed at identifying a comprehensive chemosensory signal transduction pathway in PPNs.
(© 2023. The Author(s).)
Databáze: MEDLINE
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