Environmental monitoring of SARS-CoV-2 in the metropolitan area of Porto Alegre, Rio Grande do Sul (RS), Brazil.

Autor: Dutra LB; Laboratory of Molecular Microbiology and Cytotoxicity, Health Sciences Institute, Feevale University, ERS 239 n° 2755, Novo Hamburgo, RS, CEP 93352-000, Brazil., Stein JF; Laboratory of Molecular Microbiology and Cytotoxicity, Health Sciences Institute, Feevale University, ERS 239 n° 2755, Novo Hamburgo, RS, CEP 93352-000, Brazil., da Rocha BS; Laboratory of Molecular Microbiology and Cytotoxicity, Health Sciences Institute, Feevale University, ERS 239 n° 2755, Novo Hamburgo, RS, CEP 93352-000, Brazil., Berger A; Division of Laboratories, Henrique Luis Roessler State Foundation for Environmental Protection (FEPAM), Porto Alegre, RS, CEP 90020-021, Brazil., de Souza BA; Division of Laboratories, Henrique Luis Roessler State Foundation for Environmental Protection (FEPAM), Porto Alegre, RS, CEP 90020-021, Brazil., Prandi BA; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, CEP 90050-170, Brazil., Mangini AT; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, CEP 90050-170, Brazil., Jarenkow A; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, RS, CEP 90119-900, Brazil., Campos AAS; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, RS, CEP 90119-900, Brazil., Fan FM; Hydraulic Research Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, CEP 91501-970, Brazil., de Almeida Silva MC; Hydraulic Research Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, CEP 91501-970, Brazil., Lipp-Nissinen KH; Division of Laboratories, Henrique Luis Roessler State Foundation for Environmental Protection (FEPAM), Porto Alegre, RS, CEP 90020-021, Brazil., Loncan MR; Division of Laboratories, Henrique Luis Roessler State Foundation for Environmental Protection (FEPAM), Porto Alegre, RS, CEP 90020-021, Brazil., Augusto MR; Center of Engineering, Modelling and Applied Social Sciences (CECS), Federal University of ABC (UFABC), Santo Andre, SP, CEP 09210-580, Brazil., Franco AC; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, CEP 90050-170, Brazil., de Freitas Bueno R; Center of Engineering, Modelling and Applied Social Sciences (CECS), Federal University of ABC (UFABC), Santo Andre, SP, CEP 09210-580, Brazil., Rigotto C; Laboratory of Molecular Microbiology and Cytotoxicity, Health Sciences Institute, Feevale University, ERS 239 n° 2755, Novo Hamburgo, RS, CEP 93352-000, Brazil. rigottocarol@gmail.com.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2024 Jan; Vol. 31 (2), pp. 2129-2144. Date of Electronic Publication: 2023 Dec 06.
DOI: 10.1007/s11356-023-31081-8
Abstrakt: Since starts the coronavirus disease 2019 (COVID-19) pandemic identified the presence of genomic fragments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in various environmental matrices: domestic sewage, surface waters, and contaminated freshwater. Environmental monitoring of SARS-CoV-2 is a tool for evaluating trend curves over the months, compared to several clinical cases of the disease. The objective of this study was to monitor the SARS-CoV-2 in environmental samples collected in different sites in a metropolitan area of Porto Alegre, Southern Brazil. During 10 months from 2020 to 2021, 300 samples were collected weekly and biweekly from nine points located in 3 cities: one point from a wastewater treatment plant (WWTP) in São Leopoldo (fortnightly collection), two points in Dilúvio Stream in Porto Alegre (fortnightly collection), two points in Pampa and Luiz Rau Streams (weekly collection), and two points in public fountains (fortnightly collection) in Novo Hamburgo. After collection, samples were concentrated by ultracentrifugation, and viral nucleic acids were extracted using MagMax® Core Nucleic Acid Purifications kits and submitted to RT-qPCR, using E, N1, and N2 gene targets of SARS-CoV-2. Only 7% (3/41) samples from public fountains were positive, with a mean viral load (VL) of SARS-CoV-2 RNA of 5.02 × 10 1 gc/l (2.41~8.59 × 10 1 gc/l), while the streams had average VL of 7.43 × 10 5 gc/l (Pampa), 7.06 × 10 5 gc/l (Luiz Rau), 2.01 × 10 5 gc/l (Dilúvio), and 4.46 × 10 5 cg/l (WWTP). The results showed varying levels of viral presence in different sample types, with a demonstrated correlation between environmental viral load and clinical COVID-19 cases. These findings contribute to understanding virus persistence and transmission pathways in the environment. Continuous monitoring, especially in less developed regions, is crucial for early detection of vaccine resistance, new variants, and potential COVID-19 resurgence.
(© 2023. The Author(s).)
Databáze: MEDLINE
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