Antioxidant effects of LEDT in dystrophic muscle cells: involvement of PGC-1α and UCP-3 pathways.

Autor: da Rocha GL; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil., Guimarães DSPSF; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil.; Obesity and Comorbidities Research Center (OCRC), Campinas, Brazil., da Cruz MV; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil.; Obesity and Comorbidities Research Center (OCRC), Campinas, Brazil., Mizobuti DS; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil., da Silva HNM; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil., Pereira ECL; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil.; Faculty of Ceilândia, University of Brasília (UnB), Brasília, Brazil., Silveira LR; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil.; Obesity and Comorbidities Research Center (OCRC), Campinas, Brazil., Minatel E; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970862, Brazil. minatel@unicamp.br.
Jazyk: angličtina
Zdroj: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2024 Jan; Vol. 23 (1), pp. 107-118. Date of Electronic Publication: 2023 Dec 06.
DOI: 10.1007/s43630-023-00506-1
Abstrakt: Purpose: Reactive oxygen species and mitochondrial dysfunction play a crucial role in the pathophysiology of Duchenne muscular dystrophy (DMD). The light-emitting diode therapy (LEDT) showed beneficial effects on the dystrophic muscles. However, the mechanisms of this therapy influence the molecular pathways in the dystrophic muscles, particularly related to antioxidant effects, which still needs to be elucidated. The current study provides muscle cell-specific insights into the effect of LEDT, 48 h post-irradiation, on oxidative stress and mitochondrial parameters in the dystrophic primary muscle cells in culture.
Methods: Dystrophic primary muscle cells were submitted to LEDT, at multiple wavelengths (420 nm, 470 nm, 660 nm and 850 nm), 0.5 J dose, and evaluated after 48 h based on oxidative stress markers, antioxidant enzymatic system and biogenesis, and functional mitochondrial parameters.
Results: The mdx muscle cells treated with LEDT showed a significant reduction of H 2 O 2 production and 4-HNE, catalase, SOD-2, and GR levels. Upregulation of UCP3 was observed with all wavelengths while upregulation of PGC-1α and a slight upregulation of electron transport chain complexes III and V was only observed following 850 nm LEDT. In addition, the mitochondrial membrane potential and mitochondrial mass mostly tended to be increased following LEDT, while parameters like O 2 ·- production tended to be decreased.
Conclusion: The data shown here highlight the potential of LEDT as a therapeutic agent for DMD through its antioxidant action by modulating PGC-1α and UCP3 levels.
(© 2023. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.)
Databáze: MEDLINE
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