Comprehensive metabolomics of Philippine Stichopus cf. horrens reveals diverse classes of valuable small molecules for biomedical applications.

Autor: Torreno VPM; The Marine Science Institute, University of the Philippines, Diliman, Quezon City, Philippines., Molino RJEJ; Institute of Chemistry, University of the Philippines, Diliman, Quezon City, Philippines., Junio HA; Institute of Chemistry, University of the Philippines, Diliman, Quezon City, Philippines., Yu ET; The Marine Science Institute, University of the Philippines, Diliman, Quezon City, Philippines.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Dec 06; Vol. 18 (12), pp. e0294535. Date of Electronic Publication: 2023 Dec 06 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0294535
Abstrakt: Stichopus cf. horrens is an economically important sea cucumber species in Southeast Asia due to their presumed nutritional and medicinal benefits. However, compared to other sea cucumbers such as Apostichopus japonicus, there are no biochemical studies on which compounds contribute to the purported bioactivities of S. cf. horrens. To address this, a high-throughput characterization of the global metabolite profile of the species was performed through LC-MS/MS experiments and utilizing open-access platforms such as GNPS, XCMS, and metaboAnalyst. Bioinformatics-based molecular networking and chemometrics revealed the abundance of phospholipids such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), and phosphatidylserines (PSs) in the crude samples. Body wall extracts were observed to have higher levels of structural, diacylated PCs, while the viscera have higher relative abundance of single-tail PCs and PEs that could be involved in digestion via nutrient absorption and transport for sea cucumbers. PEs and sphingolipids could also be implicated in the ecological response and morphological transformations of S. cf. horrens in the presence of predatory and other environmental stress. Interestingly, terpenoid glycosides and saponins with reported anti-cancer benefits were significantly localized in the body wall. The sulfated alkanes and sterols present in S. cf. horrens bear similarity to known kairomones and other signaling molecules. All in all, the results provide a baseline metabolomic profile of S. cf. horrens that may further be used for comparative and exploratory studies and suggest the untapped potential of S. cf. horrens as a source of bioactive molecules.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Torreno et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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