Autor: |
Silva MM; Universidade Federal de Pernambuco, Centro Acadêmico de Vitória, Núcleo de Biologia, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil., Campos TA; Centro Tecnológico do Nordeste, Av. Prof. Luís Freire, 1, Cidade Universitária, 50740-545 Recife, PE, Brazil., Cavalcanti IMF; Universidade Federal de Pernambuco, Centro Acadêmico de Vitória, Núcleo de Biologia, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil.; Universidade Federal de Pernambuco, Instituto Keizo-Asami (iLIKA), Av. Prof. Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil., Oliveira IS; Universidade Federal de Pernambuco, Centro Acadêmico de Vitória, Núcleo de Biologia, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil., Pérez CD; Universidade Federal de Pernambuco, Centro Acadêmico de Vitória, Núcleo de Biologia, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil., Silva RAD; Universidade Federal de Pernambuco, Instituto Keizo-Asami (iLIKA), Av. Prof. Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil., Wanderley MSO; Universidade de Pernambuco, Campus Santo Amaro, Instituto de Ciências Biológicas, Arnóbio Marques, 310, Santo Amaro, 50100-130 Recife, PE, Brazil., Santos NPS; Universidade Federal de Pernambuco, Centro Acadêmico de Vitória, Núcleo de Biologia, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil. |
Abstrakt: |
Mucus, produced by Palythoa caribaeorum has been popularly reported due to healing, anti-inflammatory, and analgesic effects. However, biochemical and pharmacological properties of this mucus remains unexplored. Therefore, the present study aimed to study its proteome profile by 2DE electrophoresis and MALDI-TOF. Furthermore, it was evaluated the cytotoxic, antibacterial, and antioxidant activities of the mucus and from its protein extract (PE). Proteomics study identified14 proteins including proteins involved in the process of tissue regeneration and death of tumor cells. The PE exhibited cell viability below 50% in the MCF-7 and S-180 strains. It showed IC50 of 6.9 μg/mL for the J774 lineage, and also, favored the cellular growth of fibroblasts. Furthermore, PE revealed activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Staphylococcus epidermidis (MIC of 250 μg/mL). These findings revealed the mucus produced by Palythoa caribaeorum with biological activities, offering alternative therapies for the treatment of cancer and as a potential antibacterial agent. |