| Autor: |
Muniz AR; Universidade Estadual de Feira de Santana - UEFS, Departamento de Ciências Biológicas, Feira de Santana, BA, Brasil., Jesus LS; Universidade do Estado da Bahia - UNEB, Departamento de Ciências Exatas e da Terra, Alagoinhas, BA, Brasil., Marques EJ; Universidade do Estado da Bahia - UNEB, Departamento de Ciências Exatas e da Terra, Alagoinhas, BA, Brasil., Vale VLC; Universidade do Estado da Bahia - UNEB, Departamento de Ciências Exatas e da Terra, Alagoinhas, BA, Brasil., Valente EB; Universidade Estadual de Feira de Santana - UEFS, Departamento de Ciências Biológicas, Feira de Santana, BA, Brasil. |
| Jazyk: |
angličtina |
| Zdroj: |
Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2023 Dec 04; Vol. 83, pp. e273243. Date of Electronic Publication: 2023 Dec 04 (Print Publication: 2023). |
| DOI: |
10.1590/1519-6984.273243 |
| Abstrakt: |
The indiscriminate use of allopathic drugs has selected resistant bacterial and fungal populations which represents a severe public health problem worldwide. On the other hand, plants are in a prominent position due to the capability to synthesize structurally complex bioactive metabolites that can be an alternative to resistant microorganisms' control. In this work, we evaluated the chemical composition and the antimicrobial, antioxidant, and cytotoxic potential of the fractionated extract of C. savannarum in ethyl acetate. The extract of C. savannarum was divided into 12 fractions that were submitted to phytochemical screening, minimum inhibitory concentration (MIC), reduction of 1,1-diphenyl-2-picrylhydrazine (DPPH), and hemolytic activity of sheep erythrocytes assays. During the investigation, all extract fractions presented alkaloids, triterpenoids, steroids, and phenolic compounds in qualitative analyses, while in the quantitative evaluation, we observed the presence of both phenols and flavonoids in these fractions. Among the fraction, the highest phenolic content was observed in the Cs23-24 fraction (2.480 mg EAG/g), while the Cs31-34 fractions presented the highest amount of flavonoid (182.25 μg EQ/100 mg). Nine of the 12 fractions of the moss species' extract showed antimicrobial action Against Gram-positive bacteria: Bacillus cereus, Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis; Gram-negative bacteria: Pseudomonas aeruginosa; and also antifungal activity against Candida albicans and Candida glabata. The cytotoxic assay demonstrated that the tested fractions did not induce hemolysis at concentrations 10 and 100(µG/ML). In the antioxidant evaluation, the Cs55-69 fractions were the ones that presented the highest scavenging activity (57, 0%) followed by the Cs45-54 fraction (42.7%). Overall, the evaluation of the biological potential of the fractionated extracts of Campylopus savannarum showed promising data, in the search for natural antimicrobial compounds. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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