| Autor: |
Udduttula A; School of Engineering, Newcastle University, Newcastle Upon Tyne NE1 7RU, U.K.; Centre of Biomaterials, Cellular & Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore, TN 632014, India., Jakubovics N; School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, U.K., Khan I; Biomet UK Healthcare Ltd, Stella Building, Windmill Hill Business Park, Swindon SN5 6NX, U.K., Pontiroli L; Biomet UK Healthcare Ltd, Stella Building, Windmill Hill Business Park, Swindon SN5 6NX, U.K., Rankin KS; Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K., Gentile P; School of Engineering, Newcastle University, Newcastle Upon Tyne NE1 7RU, U.K., Ferreira AM; School of Engineering, Newcastle University, Newcastle Upon Tyne NE1 7RU, U.K. |
| Abstrakt: |
Implant-associated severe infections can result in catastrophic implant failures; thus, advanced antibacterial coatings are needed to combat infections. This study focuses on harnessing nature-inspired self-assembly of extracellular matrix (ECM)-like coatings on Ti alloy with a combination of jellyfish-derived collagen (J-COLL) and hyaluronic acid (HA) using our customized automated hybrid layer-by-layer apparatus. To improve the anti-infection efficacy of coatings, we have incorporated a natural antibacterial agent methylglyoxal (MGO, a Manuka honey compound) in optimized multilayer coatings. The obtainment of MGO-loaded multilayer coatings was successfully assessed by profilometry, contact angle, attenuated total reflectance (ATR)-Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. In vitro degradation confirmed the controlled release activity of MGO with a range of concentrations from 0.90 to 2.38 mM up to 21 days. A bacterial cell culture study using Escherichia coli ( E. coli ) and Staphylococcus epidermidis ( S. epidermidis ) confirmed that the MGO incorporated within layers 7 and 9 had a favorable effect on preventing bacterial growth and colonization on their surfaces. An in vitro cytocompatibility study confirmed that MGO agents included in the layers did not affect or reduce the cellular functionalities of L929 fibroblasts. In addition, MGO-loaded layers with Immortalized Mesenchymal Stem Cells (Y201 TERT-hMSCs) were found to favor the growth and differentiation of Y201 cells and promote calcium nodule formation. Overall, these surface coatings are promising candidates for delivering antimicrobial activity with bone-inducing functions for future bone tissue engineering applications. |
