Vaping product exposure system (VaPES): a novel in vitro aerosol deposition system.

Autor: Schichlein KD; Curriculum in Toxicology & Environmental Medicine, University of NC at Chapel Hill, Chapel Hill, NC, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Love CA; Curriculum in Toxicology & Environmental Medicine, University of NC at Chapel Hill, Chapel Hill, NC, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Conolly MP; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Kurz JL; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Hickman ED; Curriculum in Toxicology & Environmental Medicine, University of NC at Chapel Hill, Chapel Hill, NC, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Clapp PW; Curriculum in Toxicology & Environmental Medicine, University of NC at Chapel Hill, Chapel Hill, NC, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Jaspers I; Curriculum in Toxicology & Environmental Medicine, University of NC at Chapel Hill, Chapel Hill, NC, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Jazyk: angličtina
Zdroj: Inhalation toxicology [Inhal Toxicol] 2023 Dec; Vol. 35 (13-14), pp. 324-332. Date of Electronic Publication: 2023 Dec 13.
DOI: 10.1080/08958378.2023.2289021
Abstrakt: Objective: Due to recent increases in the use of vaping devices, there is a high demand for research addressing the respiratory health effects of vaping products. Given the constantly changing nature of the vaping market with new devices, flavors, metals, and other chemicals rapidly emerging, there is a need for inexpensive and highly adaptable vaping device exposure systems. Here, we describe the design and validation of a novel in vitro aerosol exposure system for toxicity testing of vaping devices.
Materials and Methods: We developed an inexpensive, open-source in vitro vaping device exposure system that produces even deposition, can be adapted for different vaping devices, and allows for experiments to be performed under physiological conditions. The system was then validated with deposition testing and a representative exposure with human bronchial epithelial cells (hBECs).
Results: The Vaping Product Exposure System (VaPES) produced sufficient and uniform deposition for dose-response studies and was precise enough to observe biological responses to vaping exposures. VaPES was adapted to work with both pod and cartridge-based vaping devices.
Conclusion: We have designed and validated a novel vaping device exposure system that will eliminate the need to use high-cost commercial exposure systems, lowering the barrier to entry of physiologically relevant vaping studies.
Databáze: MEDLINE
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