Farnesol as an antifungal agent: comparisons among MTL a and MTLα haploid and diploid Candida albicans and Saccharomyces cerevisiae .

Autor:	Boone CHT; School of Biological Sciences, University of Nebraska, Lincoln, NE, United States., Parker KA; School of Biological Sciences, University of Nebraska, Lincoln, NE, United States., Gutzmann DJ; School of Biological Sciences, University of Nebraska, Lincoln, NE, United States., Atkin AL; School of Biological Sciences, University of Nebraska, Lincoln, NE, United States., Nickerson KW; School of Biological Sciences, University of Nebraska, Lincoln, NE, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2023 Nov 20; Vol. 14, pp. 1207567. Date of Electronic Publication: 2023 Nov 20 (Print Publication: 2023).
DOI:	10.3389/fphys.2023.1207567
Abstrakt:	Aims: Farnesol was identified 20 years ago in a search for Candida albicans quorum sensing molecules (QSM), but there is still uncertainty regarding many aspects of its mode of action including whether it employs farnesol transport mechanisms other than diffusion. Based on the structural similarity between farnesol and the farnesylated portion of the MTL a pheromone, we explored the effects of ploidy and mating type locus ( MTL ) on the antifungal activity of exogenous farnesol. Methods and results: We approached this question by examining five MTL a and five MTLα haploid strains with regard to their farnesol sensitivity in comparison to six heterozygous MTL a / α diploids. We examined the haploid and diploid strains for percent cell death after exposure of exponentially growing cells to 0-200 µM farnesol. The heterozygous ( MTL a /α) diploids were tolerant of exogenous farnesol whereas the MTL a and MTL α haploids were on average 2- and 4-times more sensitive, respectively. In the critical range from 10-40 µM farnesol their cell death values were in the ratio of 1:2:4. Very similar results were obtained with two matched sets of MAT a , MAT α, and MAT a /α Saccharomyces cerevisiae strains. Conclusion: We propose that the observed MTL dependence of farnesol is based on differentially regulated mechanisms of entry and efflux which determine the actual cellular concentration of farnesol. The mechanisms by which pathogens such as C. albicans tolerate the otherwise lethal effects of farnesol embrace a wide range of physiological functions, including MTL type, ubiquinone type (UQ6-UQ9), energy availability, and aerobic/anaerobic status. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Boone, Parker, Gutzmann, Atkin and Nickerson.)
Databáze:	MEDLINE
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