| Autor: |
Alcolea-Medina A; Department of Infectious Diseases, King's College London, London, UK.; Infection Sciences, Synnovis, London, UK., Snell LB; Department of Infectious Diseases, King's College London, London, UK.; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Payne L; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Alder CJ; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Turnbull JD; The National Collection of Type Cultures, United Kingdom Health Security Agency, Colindale, London, UK., Charalampous T; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Bryan L; Infection Sciences, Synnovis, London, UK., Klein JL; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Edgeworth JD; Department of Infectious Diseases, King's College London, London, UK.; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Batra R; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK., Goodman AL; Department of Infectious Diseases, King's College London, London, UK.; Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust, London, UK.; Medical Research Council Clinical Trials Unit, University College London, London, UK. |
| Abstrakt: |
A novel bacterial strain, GSTT-20 T was isolated from an infected, prosthetic endovascular graft explanted from a shepherd in London, United Kingdom. This strain was an aerobic, catalase-positive, oxidase-negative, Gram-stain-negative, motile, curved rod. It grew on blood agar, chocolate agar and MacConkey agar incubated at 37 °C in an aerobic environment after 48 h, appearing as yellow, mucoid colonies. Analysis of the complete 16S rRNA gene sequence showed closest similarity to Variovorax paradoxus with 99.6 % identity and Variovorax boronicumulans with 99.5 % identity. Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of single nucleotide polymorphisms within 1530 core genes showed GSTT-20 T forms a distinct lineage in the genus Variovorax of the family Comamonadaceae . In silico DNA-DNA hybridization assays against GSTT-20 T were estimated at 32.1 % for V. boronicumulans and 31.9 % for V. paradoxus . Genome similarity based on average nucleotide identity was 87.50 % when comparing GSTT-20 T to V. paradoxus . Based on these results, the strain represented a novel species for which the name Variovorax durovernensis sp. nov. was proposed. The type strain is GSTT-20 T (NCTC 14621 T =CECT 30390 T ). |
