Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis .

Autor:	Poester VR; Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.; Laboratório de Micologia (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Hidalgo JED; Laboratório de Micologia (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Jardim LS; Laboratório de Micologia (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Trápaga MR; Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.; Laboratório de Micologia (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Rabello VBS; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro state, Brazil., Almeida-Paes R; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro state, Brazil., Zancope-Oliveira RM; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro state, Brazil., Xavier MO; Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.; Laboratório de Micologia (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Nov 30; Vol. 11, pp. e16443. Date of Electronic Publication: 2023 Nov 30 (Print Publication: 2023).
DOI:	10.7717/peerj.16443
Abstrakt:	Background: Sporotrichosis caused by Sporothrix brasiliensis is a globally emerging infectious disease with limited therapeutic options. Thus, we aimed to evaluate the in vitro activity of amlodipine (AML) and lufenuron (LUF) alone and their interaction with itraconazole (ITZ), the first-choice drug against S. brasiliensis . Methods: Twenty clinical isolates of S. brasiliensis from two hyperendemic regions were tested through a microdilution assay to evaluate the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of AML and LUF. Checkerboard assay was performed with 10 isolates for both drug interactions with ITZ. Results: AML showed inhibitory and fungicidal activity against all isolates included, with MIC values ranging from 32 to 256 µg/mL, and MFC from 64 to 256 µg/mL. However, none of the S. brasiliensis isolates were inhibited by the highest soluble concentration of LUF (MIC >64 µg/mL for all strains). Synergic interaction of AML and LUF with ITZ occurred in 50% and 40% of the isolates tested, without any antagonistic effects. Conclusion: Both repurposing drugs evaluated in our study showed a promising in vitro activity, especially in synergy with ITZ against S. brasiliensis , warranting future in vivo investigations regarding its activity. Competing Interests: The authors declare there are no competing interests. (©2023 Poester et al.)
Databáze:	MEDLINE
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