Functional Inhibition of Katanin Affects Synaptic Plasticity.

Autor:	Lombino FL; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Schwarz JR; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Pechmann Y; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Schweizer M; Core Facility of Morphology and Electron Microscopy, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Jark R; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Stange O; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Glatzel M; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, Hamburg 20246, Germany., Gee CE; Institute of Synaptic Physiology, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Hamburg 20251, Germany., Hausrat TJ; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany., Gromova KV; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany matthias.kneussel@zmnh.uni-hamburg.de kira.gromova@zmnh.uni-hamburg.de., Kneussel M; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, Hamburg 20251, Germany matthias.kneussel@zmnh.uni-hamburg.de kira.gromova@zmnh.uni-hamburg.de.
Jazyk:	angličtina
Zdroj:	The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2024 Mar 27; Vol. 44 (13). Date of Electronic Publication: 2024 Mar 27.
DOI:	10.1523/JNEUROSCI.0374-23.2023
Abstrakt:	Dynamic microtubules critically regulate synaptic functions, but the role of microtubule severing in these processes is barely understood. Katanin is a neuronally expressed microtubule-severing complex regulating microtubule number and length in cell division or neurogenesis; however, its potential role in synaptic functions has remained unknown. Studying mice from both sexes, we found that katanin is abundant in neuronal dendrites and can be detected at individual excitatory spine synapses. Overexpression of a dominant-negative ATPase-deficient katanin subunit to functionally inhibit severing alters the growth of microtubules in dendrites, specifically at premature but not mature neuronal stages without affecting spine density. Notably, interference with katanin function prevented structural spine remodeling following single synapse glutamate uncaging and significantly affected the potentiation of AMPA-receptor-mediated excitatory currents after chemical induction of long-term potentiation. Furthermore, katanin inhibition reduced the invasion of microtubules into fully developed spines. Our data demonstrate that katanin-mediated microtubule severing regulates structural and functional plasticity at synaptic sites. Competing Interests: The authors declare no competing financial interests. (Copyright © 2024 Lombino et al.)
Databáze:	MEDLINE
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