Transmembrane coupling of liquid-like protein condensates.

Autor: Lee Y; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA., Park S; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea., Yuan F; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA., Hayden CC; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA., Wang L; Department of Biochemistry and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA., Lafer EM; Department of Biochemistry and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA., Choi SQ; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea., Stachowiak JC; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA. jcstach@austin.utexas.edu.; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA. jcstach@austin.utexas.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Dec 04; Vol. 14 (1), pp. 8015. Date of Electronic Publication: 2023 Dec 04.
DOI: 10.1038/s41467-023-43332-w
Abstrakt: Liquid-liquid phase separation of proteins occurs on both surfaces of cellular membranes during diverse physiological processes. In vitro reconstitution could provide insight into the mechanisms underlying these events. However, most existing reconstitution techniques provide access to only one membrane surface, making it difficult to probe transmembrane phenomena. To study protein phase separation simultaneously on both membrane surfaces, we developed an array of freestanding planar lipid membranes. Interestingly, we observed that liquid-like protein condensates on one side of the membrane colocalized with those on the other side, resulting in transmembrane coupling. Our results, based on lipid probe partitioning and mobility of lipids, suggest that protein condensates locally reorganize membrane lipids, a process which could be explained by multiple effects. These findings suggest a mechanism by which signals originating on one side of a biological membrane, triggered by protein phase separation, can be transferred to the opposite side.
(© 2023. The Author(s).)
Databáze: MEDLINE
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