Potential of yeasts as biocontrol agents against Fusarium graminearum in vitro and on corn.

Autor:	de Oliveira AJ; Department of Biochemistry and Biotechnology, State University of Londrina, P.O. box 10.011, 86057-970 Londrina, Paraná, Brazil., Ono MA; Department of Pathological Sciences, State University of Londrina, P.O. box 10.011, 86057-970 Londrina, Paraná, Brazil., Suguiura IMS; Department of Pathological Sciences, State University of Londrina, P.O. box 10.011, 86057-970 Londrina, Paraná, Brazil., Zucareli C; Department of Agronomy, State University of Londrina, P.O. box 10.011, 86057-970 Londrina, Paraná, Brazil., Garcia EB; Department of Agronomy, State University of Londrina, P.O. box 10.011, 86057-970 Londrina, Paraná, Brazil., Olchanheski LR; Department of Structural, Molecular and Genetic Biology, State University of Ponta Grossa, 84030-900 Ponta Grossa, Paraná, Brazil., Ono EYS; Department of Biochemistry and Biotechnology, State University of Londrina, P.O. box 10.011, 86057-970 Londrina, Paraná, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2023 Dec 01; Vol. 134 (12).
DOI:	10.1093/jambio/lxad296
Abstrakt:	Aims: The antifungal effect of the yeast species Kluyveromyces marxianus, Meyerozyma caribbica, and Wickerhamomyces anomalus was evaluated against two Fusarium graminearum strains (FRS 26 and FSP 27) in vitro and on corn seeds. Methods and Results: The antifungal effect of the yeasts against F. graminearum was evaluated using scanning electron microscopy and extracellular chitinase and glucanase production to further elucidate the biocontrol mode of action. In addition, the germination percentage and vigor test were investigated after applying yeast on corn seeds. All the yeast strains inhibited fungal growth in vitro (57.4%-100.0%) and on corn seeds (18.9%-87.2%). In co-culture with antagonistic yeasts, F. graminearum showed collapsed hyphae and turgidity loss, which could be related to the ability of yeasts to produce chitinases and glucanases. The three yeasts did not affect the seed corn germination, and W. anomalus and M. caribbica increased corn seed growth parameters (germination percentage, shoot and root length, and shoot dry weight). Conclusion: Meyerozyma caribbica and W. anomalus showed satisfactory F. graminearum growth inhibition rates and did not affect seed growth parameters. Further studies are required to evaluate the application of these yeasts to the crop in the field. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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