LPS-Induced Neuron Cell Apoptosis through TNF-α and Cytochrome c Expression in Dental Pulp.
| Autor: | Sampoerno G; Department of Conservative Dentistry, Faculty of Dental Medicine, Univeristas Airlangga, Surabaya, Indonesia., Supriyanto E; Conservative Dentistry Specialist Program, Faculty of Dental Medicine, Univeristas Airlangga, Surabaya, Indonesia., Wahjuningrum DA; Department of Conservative Dentistry, Faculty of Dental Medicine, Univeristas Airlangga, Surabaya, Indonesia., Larasati AA; Conservative Dentistry Specialist Program, Faculty of Dental Medicine, Univeristas Airlangga, Surabaya, Indonesia., Ardiani D; Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Surboyo MDC; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Bhardwaj A; Department of Conservative Dentistry and Endodontic, Collage of Dental Science and Hospital, Rau, Indore, Madhya Pradesh, India., Ates AA; Department of Endodontic, Faculty of Dentistry, Istinye University, Istanbul, Turkiye. |
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| Jazyk: | angličtina |
| Zdroj: | European journal of dentistry [Eur J Dent] 2024 May; Vol. 18 (2), pp. 604-609. Date of Electronic Publication: 2023 Dec 04. |
| DOI: | 10.1055/s-0043-1774329 |
| Abstrakt: | Objectives: Inflammation of the dental pulp tissue caused by bacteria, creating an immunology response of death of the dental pulp, is called apoptosis. The Porphyromonas gingivalis that cause apoptosis is lipopolysaccharide (LPS) through toll-like receptor (TLR) via two different mechanisms, intracellular and extracellular pathways. This study analyzed the role of LPS exposure of neuron cells, tumor necrosis factor-α (TNF-α), and cytochrome c (cyt-c) expression in the dental pulp to predict the possible mechanism of apoptosis. Materials and Methods: The lower tooth of Sprague Dawley rats was opened and exposed to LPS for 48 hours. Then the neuron cell analyzed histopathology using hematoxylin-eosin, whereas the TNF-α and cyt-c expression with indirect immunohistochemistry using a light microscope. The relationship between neuron cells with TNF-α and cyt-c was analyzed using stepwise regression linear analysis. Result: The LPS exposure showed a lower number of neuron cells and had a relationship with TNF-α expression but not with cyt-c, while compared with control, both TNF-α and cyt-c expression were higher in neuron cells. Conclusion: LPS exposure in dental pulp is possible to stimulate the apoptosis process through extracellular pathways marked by higher TNF-α expression. Competing Interests: None declared. (The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).) |
| Databáze: | MEDLINE |
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